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Characterization of Cellulose Synthase A (CESA) Gene Family in Eudicots

机译：避免纤维素合酶A（CESA）基因家族的特征

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Cellulose synthase A (CESA) is a key enzyme involved in the complex process of plant cell wall biosynthesis, and it remains a productive subject for research. We employed systems biology approaches to explore structural diversity of eudicot CESAs by exon-intron organization, mode of duplication, synteny, and splice site analyses. Using a combined phylogenetics and comparative genomics approach coupled with co-expression networks we reconciled the evolution of cellulose synthase gene family in eudicots and found that the basic forms of CESA proteins are retained in angiosperms. Duplications have played an important role in expansion of CESA gene family members in eudicots. Co-expression networks showed that primary and secondary cell wall modules are duplicated in eudicots. We also identified 230 simple sequence repeat markers in 103 eudicot CESAs. The 13 identified conserved motifs in eudicots will provide a basis for gene identification and functional characterization in other plants. Furthermore, we characterized (in silico) eudicot CESAs against senescence and found that expression levels of CESAs decreased during leaf senescence.
机译：纤维素合酶A（CESA）是植物细胞壁生物合成的复杂过程中涉及的关键酶，并且它仍然是研究的生产受试者。我们采用了系统生物学方法来探讨外显子组织，复制，同步和剪接站点分析的外显科组织的结构多样性。使用组合的系统源和比较基因组学与联合表达网络相结合，我们与避开的纤维素合成酶基因家族的演变进行了调整，发现CESA蛋白的基本形式保留在Anuiaperms中。重复性在拒于eudicots的CESA基因家族成员的扩展中发挥了重要作用。共表达网络表明，初级和次级电池壁模块在eudicots中复制。我们还在103 eudicot CESAS中识别了230个简单的序列重复标记。拒于的13个鉴定的保守基序将为其他植物的基因鉴定和功能表征提供基础。此外，我们以衰老的特征（在Silico）eudicot cesas，发现叶片在叶片衰老期间Cesas的表达水平降低。

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