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首页> 外文期刊>Biochemical Genetics >Upregulation of Pro-inflammatory Cytokine Genes by Parvovirus B19 in Human Bone Marrow Mesenchymal Stem Cells
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Upregulation of Pro-inflammatory Cytokine Genes by Parvovirus B19 in Human Bone Marrow Mesenchymal Stem Cells

机译：Parvovirus B19在人骨髓间充质干细胞中的促炎细胞因子基因的上调

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摘要

Chronic inflammation plays a prominent role in cancer initiation and development. On the other hand, the Inflammation can be established by a number of factors such as viral infections. Parvovirus B19 (B19V) is a pathogen with widespread infection, which infects bone marrow erythroid progenitor cells. It has been shown that B19V can also enter human bone marrow mesenchymal stem cells (BM-MSCs). In this study, we hypothesized that BM-MSCs as the main cellular component of bone marrow niche may be induced to secret pro-inflammatory cytokines after B19V infection. BM-MSCs were cultured up to passage 3. The cells were then subjected to nucleofection to transfer a plasmid containing B19V genome. After 36 h, total RNA was extracted and the expression levels of IL-1 beta, IL-6, TNF-alpha and NF-kappa B genes were examined using qRT-PCR. Data analysis showed the significant increase in expression levels of all studied genes in the B19V-transfected cells (P<0.05). Although further researches are required, our findings for the first time suggest the importance of B19V infection to establish an inflammatory microenvironment in the bone marrow and its involvement in inflammation-related diseases. Finally, based on our results, molecular assay to diagnose B19V infection of BM-MSCs prior to stem cell therapy is strongly recommended.
机译：慢性炎症在癌症启动和发展中起着突出的作用。另一方面，可以通过诸如病毒感染的多种因素来建立炎症。 Parvovirus B19（B19V）是一种具有广泛感染的病原体，其感染骨髓红细胞祖细胞。已经表明B19V也可以进入人骨髓间充质干细胞（BM-MSCs）。在这项研究中，我们假设BM-MSCs作为骨髓利基的主要细胞成分可以在B19V感染后诱导秘密促炎细胞因子。将BM-MSCs培养至通过3.然后对细胞进行核原子以转移含有B19V基因组的质粒。 36小时后，用QRT-PCR检查IL-1β，IL-6，TNF-α和NF-κB和NF-κB基因的总RNA。数据分析显示B19V转染细胞中所有研究基因的表达水平的显着增加（P <0.05）。虽然需要进一步的研究，但我们首次发现的结果表明B19V感染在骨髓中建立炎症微环境的重要性及其参与炎症相关疾病。最后，基于我们的结果，强烈建议强烈建议在干细胞疗法之前诊断B19V感染B19V感染的分子测定。

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