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首页> 外文期刊>Biological chemistry >Homo- and heterotypic interactions between Pss proteins involved in the exopolysaccharide transport system in Rhizobium leguminosarum bv. trifolii
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Homo- and heterotypic interactions between Pss proteins involved in the exopolysaccharide transport system in Rhizobium leguminosarum bv. trifolii

机译：PSS蛋白在乳杆菌BV中的PSS蛋白质之间的同源和异相相互作用。 trifolii.

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摘要

Rhizobium leguminosarum produces large amounts of exopolysaccharide (EPS) that has been shown to be an important determinant of successful nitrogen-fixing symbiosis with legume plants. EPS is assembled in a Wzx/Wzy-dependent manner, and proteins involved in the process are proposed to form a complex that enables coupling the synthesis of EPS subunits with their polymerization and transport. Pss proteins, which are encoded within the chromosomal polysaccharide synthesis cluster of Rhizobium leguminosarum bv. trifolii TA1, were subjected to interaction analysis. PssN was shown to form multimeric complexes in the outer membrane and interact with the extracellular PssO protein and the inner membrane oligomeric PssP co-polymerase. PssO was demonstrated to form oligomers in the presence of the cross-linker. Bacterial two-hybrid analysis showed that PssP interacts with PssL and PssT, counterparts of Gram-negative bacteria Wzx and Wzy proteins. Membrane topology of PssT is discussed in the context of its plausible Wzy-like polymerase activity, interactions with PssP and a possible impact of these interactions on EPS polymerization and chain length determination. The importance of protein-protein and putative protein-polysaccharide interactions in EPS transport is discussed. A topology model for the EPS transport system, with highlights on localization, functions and interactions between the Pss proteins, is proposed.
机译：Rhizobium Leguminosarum产生大量的潜水糖（EPS），已被证明是用豆科植物成功成功氮素固定共生的重要决定因素。 EPS以WZX / WZY依赖性的方式组装，并提出了参与该方法的蛋白质以形成一种复合物，使得能够通过它们的聚合和转运来偶联EPS亚基的合成。 PSS蛋白质，其在Rexobium Molecumosarum BV的染色体多糖合成簇内编码。进行Trifolii Ta1，进行相互作用分析。显示PSSN在外膜中形成多聚体复合物，并与细胞外PSSO蛋白和内膜低聚PSSP共聚物相互作用。证明PSSO在交联剂存在下形成低聚物。细菌双杂化分析表明，PSSP与PSSL和PSST相互作用，革兰氏阴性细菌WZX和WZY蛋白的对应物。在其合理的WZY的聚合酶活性的背景下讨论了PSST的膜拓扑，与PSSP的相互作用以及这些相互作用对EPS聚合和链长测定的可能影响。讨论了蛋白质 - 蛋白质和推定蛋白 - 多糖相互作用在EPS运输中的重要性。提出了一种拓扑模型，用于突出显示PSS蛋白之间的定位，功能和相互作用。

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