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首页> 外文期刊>Biological chemistry >Homo- and heterotypic interactions between Pss proteins involved in the exopolysaccharide transport system in Rhizobium leguminosarum bv. trifolii
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Homo- and heterotypic interactions between Pss proteins involved in the exopolysaccharide transport system in Rhizobium leguminosarum bv. trifolii

机译：豆科根瘤菌胞外多糖转运系统中的Pss蛋白之间的同型和异型相互作用。三叶草

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摘要

Rhizobium leguminosarum produces large amounts of exopolysaccharide (EPS) that has been shown to be an important determinant of successful nitrogen-fixing symbiosis with legume plants. EPS is assembled in a Wzx/Wzy-dependent manner, and proteins involved in the process are proposed to form a complex that enables coupling the synthesis of EPS subunits with their polymerization and transport. Pss proteins, which are encoded within the chromosomal polysaccharide synthesis cluster of Rhizobium leguminosarum bv. trifolii TA1, were subjected to interaction analysis. PssN was shown to form multimeric complexes in the outer membrane and interact with the extracellular PssO protein and the inner membrane oligomeric PssP co-polymerase. PssO was demonstrated to form oligomers in the presence of the cross-linker. Bacterial two-hybrid analysis showed that PssP interacts with PssL and PssT, counterparts of Gram-negative bacteria Wzx and Wzy proteins. Membrane topology of PssT is discussed in the context of its plausible Wzy-like polymerase activity, interactions with PssP and a possible impact of these interactions on EPS polymerization and chain length determination. The importance of protein-protein and putative protein-polysaccharide interactions in EPS transport is discussed. A topology model for the EPS transport system, with highlights on localization, functions and interactions between the Pss proteins, is proposed.
机译：豆科根瘤菌会产生大量胞外多糖（EPS），已被证明是豆类植物成功固氮共生的重要决定因素。 EPS以Wzx / Wzy依赖的方式组装，并且提出了参与该过程的蛋白质以形成复合物，该复合物使EPS亚基的合成与其聚合和运输相结合。 Pss蛋白，在豆科根瘤菌的染色体多糖合成簇中编码。 Trifolii TA1，进行了相互作用分析。 PssN已显示在外膜中形成多聚体复合物，并与细胞外PssO蛋白和内膜低聚PssP共聚酶相互作用。 PssO被证明在交联剂的存在下形成低聚物。细菌两杂交分析表明，PssP与革兰氏阴性细菌Wzx和Wzy蛋白的对应物PssL和PssT相互作用。在PssT的膜拓扑学方面，以其可能的Wzy样聚合酶活性，与PssP的相互作用以及这些相互作用对EPS聚合和链长确定的可能影响为背景进行了讨论。讨论了蛋白质-蛋白质和推定的蛋白质-多糖相互作用在EPS转运中的重要性。提出了EPS运输系统的拓扑模型，重点介绍了Pss蛋白之间的定位，功能和相互作用。

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