• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry and Cell Biology >Effects of lactoferrin on the viability and the encystment of Acanthamoeba trophozoites
【24h】

Effects of lactoferrin on the viability and the encystment of Acanthamoeba trophozoites

机译：乳铁蛋白对可行性和刺槐滋生蛋白的封口的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Acanthamoeba clinical-isolate trophozoites, which cause severe keratitis. Most of the risk factor for Acanthamoeba keratitis is from wearing soft contact lenses (SCLs). Acanthamoeba trophozoites were incubated in bovine LF (bLF) solution, and the ratios of viability and encystment were determined with microscopic analysis of cyst formation. The amoebicidal effect of bLF was assessed by Trypan blue assay. The ratios of viable cells in the presence of iron-free bLF (apo-bLF), native-bLF, and iron-saturated bLF (Fe-bLF) at the concentration of 10 μmol/L for 60 min were 7.7% ± 4.6%, 80.7% ± 10.1%, and 97.3% ± 1.5%, respectively. Apo-bLF showed potent amoebicidal effect against Acanthamoeba trophozoites, but Fe-bLF did not have this effect. After treating with apo-bLF, most dead cells were nonglobular forms of trophozoites but not cystic forms. Encystment of Acanthamoeba was assessed by the sarkosyl-calcofluor white assay. The encystment ratios treated with 0.5% propylene glycol (positive control) and 10 μmol/L apo-bLF for 24 h were 96.12% ± 10.6% and 0.47% ± 0.5%, respectively. These results suggest that the amoebicidal effect of apo-bLF without encystment might lead to the prevention of contamination of Acanthamoeba in SCL stock cases.]]>
机译：Acanthamoeba临床 - 分离滋养体，引起严重的角膜炎。 Acanthamoeba角膜炎的大多数风险因素来自佩戴软隐形眼镜（SCL）。在牛LF（BLF）溶液中孵育Acanthamoeba滋养体，并测定可行性和封口的比例，并用囊肿形成的微观分析测定。通过台盼蓝测定评估BLF的Amoybicidal效果。在10μmol/ L浓度为60分钟的无铁BLF（APO-BLF），天然 - BLF和铁饱和BLF（Fe-BLF）中的活细胞的比率为7.7％±4.6％ ，80.7％±10.1％，分别为97.3％±1.5％。 APO-BLF对Acanthamoeba滋养本质表现出有效的amoebicidal效应，但Fe-Blf没有这种效果。在用APO-BLF处理后，大多数死细胞都是农石形式的滋养，但不是囊性形式。通过甲糖基 - Calcofluor白色测定评估Acanthamoeba的封口。用0.5％丙二醇（阳性对照）和10μmol/ L apo-BLF处理的心脏抑制比分别为24小时，分别为96.12％±10.6％和0.47％±0.5％。这些结果表明，APO-BLF的amoybicidal效应可能导致防止SCL股票病例中Acanthamoeba的污染。]>

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号