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首页> 外文期刊>Cytokine >The effect of interleukin-6 (IL-6)/gp130 signalling on biliary epithelial cell growth, in vitro.
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The effect of interleukin-6 (IL-6)/gp130 signalling on biliary epithelial cell growth, in vitro.

机译：白细胞介素6（IL-6）/ gp130信号转导对胆管上皮细胞生长的影响。

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摘要

The effect of IL-6 on the growth of mouse biliary epithelial cells (BEC), in vitro, was tested by comparing BEC obtained IL-6-deficient mice (IL-6(-/-)) to wild-type littermate controls (IL-6(+/+)), in two different media: simple serum-free media (S-SFM), and complete serum-free media (C-SFM) containing forskolin, which stimulates BEC IL-6 production. In S-SFM, neither IL-6(+/+)nor IL-6(-/-)BEC constitutively produced IL-6 mRNA or protein, and there was no difference between IL-6(+/+)and IL-6(-/-)BEC growth. In contrast, when the BEC were maintained in C-SFM, over 48 h, the growth of IL-6(+/+)BEC was 40% greater than IL-6(-/-)BEC (P<0.006). Enhanced IL-6(+/+)BEC growth in C-SFM was associated with induced expression of IL-6 mRNA and IL-6 protein secretion into the medium, upregulation of the IL-6Ralpha (gp80) and phosphorylation of the signal transducing molecule gp130. In C-SFM, anti-IL-6 neutralizing antibodies blocked enhanced IL-6(+/+)BEC growth, whereas exogenous rhIL-6 stimulated retarded growth of IL-6(-/-)BEC. Thus, under conditions that mimic an inflammatory or stressful microenvironment in vivo, BEC produce, secrete and respond to IL-6, via upregulation and activation of the IL-6Ralpha (gp80)/gp130 signaling system in an autocrine/paracrine manner. Copyright 2000 Academic Press.
机译：通过比较获得的BEC获得的IL-6缺陷小鼠（IL-6（-/-））与野生型同窝对照（IL），测试了IL-6在体外对小鼠胆管上皮细胞（BEC）生长的影响。 IL-6（+ / +）），有两种不同的培养基：简单的无血清培养基（S-SFM）和包含福司可林的完全无血清培养基（C-SFM），可刺激BEC IL-6的产生。在S-SFM中，IL-6（+ / +）或IL-6（-/-）BEC都不组成性地产生IL-6 mRNA或蛋白质，并且IL-6（+ / +）和IL- BEC增长6（-/-）。相反，当BEC维持在C-SFM中超过48小时时，IL-6（+ / +）BEC的生长比IL-6（-/-）BEC增长40％（P <0.006）。 C-SFM中增强的IL-6（+ / +）BEC生长与介质中IL-6 mRNA和IL-6蛋白分泌的诱导表达，IL-6Ralpha（gp80）的上调以及信号转导的磷酸化有关分子gp130。在C-SFM中，抗IL-6中和抗体阻断了IL-6（+ / +）BEC的增强生长，而外源性rhIL-6刺激了IL-6（-/-）BEC的生长受阻。因此，在模拟体内炎性或应激性微环境的条件下，BEC通过以自分泌/旁分泌方式上调和激活IL-6Ralpha（gp80）/ gp130信号系统来产生，分泌和响应IL-6。版权所有2000学术出版社。

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