Copper(II) complexation by fragment of central part of FBP28 protein from Mus musculus

Joanna Makowska; Krzysztof ?amoj?; Dariusz Wyrzykowski; Wies?aw Wiczk; Lech Chmurzyński

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Copper(II) complexation by fragment of central part of FBP28 protein from Mus musculus

机译：铜（II）由亩肌肉的FBP28蛋白的中枢部分片段络合

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Steady-state and time-resolved fluorescence spectroscopy, UV spectrophotometry and isothermal titration calorimetry techniques were used to study the coordinating properties of the 17aa peptide fragment (D17) derived from the central part of the mouse formin binding protein (FBP28 with the PDB code:1E0L) towards Cu2+ions. All the measurements were run in the 2-(N-morpholino)ethanesulfonic acid buffer (20?mM, pH?6.0). Under experimental conditions the formation of the 1:1 complex of Cu2+ions with D17 is an entropy-driven process. Cu2+ions cause the static fluorescence quenching of the peptide studied through the formation of a non-fluorescent complex. Furthermore, the thermal stability of D17 was discussed based on the results obtained from differential scanning fluorimetry (nanoDSF) data.

机译：使用稳态和时间分辨荧光光谱，UV分光光度法和等温滴定热量法研究，用于研究衍生自小鼠甲蛋白结合蛋白的中心部分（FBP28与PDB代码的17AA肽片段（D17）的配位性质（FBP28： 1E0L）朝向Cu2 +离子。所有测量都在2-（N-吗啉代）乙磺酸缓冲液（20μm，pH≤6.0）中。在实验条件下，用D17的Cu 2 +离子的1：1复合物的形成是熵驱动的方法。 Cu2 +离子引起通过形成非荧光复合物研究的肽的静态荧光猝灭。此外，基于从差示扫描荧光法（NANODSF）数据获得的结果讨论了D17的热稳定性。

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来源
《Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena》 |2018年第2018期|共6页
作者
Joanna Makowska; Krzysztof ?amoj?; Dariusz Wyrzykowski; Wies?aw Wiczk; Lech Chmurzyński;
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作者单位

Faculty of Chemistry University of Gdansk;

Faculty of Chemistry University of Gdansk;

Faculty of Chemistry University of Gdansk;

Faculty of Chemistry University of Gdansk;

Faculty of Chemistry University of Gdansk;

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正文语种 eng
中图分类生物化学;
关键词
Metal-peptide binding; Fluorescence quenching; Isothermal titration calorimetry; Thermodynamic parameters; UV spectroscopy;

机译：金属肽结合;荧光猝灭;等温滴定热量;热力学参数;UV光谱;

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1. Copper(II) complexation by fragment of central part of FBP28 protein from Mus musculus [J] . Joanna Makowska, Krzysztof ?amoj?, Dariusz Wyrzykowski, Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena . 2018,第期

机译：铜（II）由亩肌肉的FBP28蛋白的中枢部分片段络合
2. Copper(II) complexes of peptide fragments of the prion protein. Conformation changes induced by copper(II) and the binding motif in C-terminal protein region [J] . Brown DR, Guantieri V, Grasso G, Journal of Inorganic Biochemistry: An Interdisciplinary Journal . 2004,第1期

机译：ion病毒蛋白肽片段的铜（II）配合物。铜（II）诱导的构象变化和C端蛋白区域的结合基序
3. Mixed metal copper(II)-nickel(II) and copper(II)-zinc(II) complexes of multihistidine peptide fragments of human prion protein [J] . Jószai V., Turi I., Kállay C., Journal of Inorganic Biochemistry: An Interdisciplinary Journal . 2012,第Null期

机译：人病毒蛋白多组氨酸肽片段的混合金属铜（II）-镍（II）和铜（II）-锌（II）配合物
4. Study of the Relationship between Mus musculus Protein Sequences and their Biological Functions [C] . Pawan Seth, Zhong-Hui Duan 2009 WASE International Conference on Information Engineering(2009年国际信息工程会议)(ICIE 2009)论文集 . 2009

机译：小家鼠蛋白质序列与其生物学功能关系的研究
5. Copper protein biomimetic studies using low molecular weight copper(I) and copper(II) complexes [D] . Lee, Dong-Heon. 1996

机译：使用低分子量铜（I）和铜（II）配合物进行铜蛋白仿生研究
6. Copper(II)-bis-Histidine Coordination Structure in Fibrillar Amyloid-β Peptide Fragment and Model Complexes Revealed by using Electron Spin Echo Envelope Modulation Spectroscopy [O] . Jessica Hernández-Guzmán, Li Sun, Anil K. Mehta, -1

机译：电子自旋回波包络调制光谱法揭示原纤维淀粉样β肽片段中铜（II）-双-组氨酸的配位结构和模型配合物
7. Investigations of copper(II) complexation by fragments of the FBP28 protein using isothermal titration (ITC) and differential scanning calorimetry (DSC) [O] . Joanna Makowska, Dariusz Wyrzykowski, Paulina Hirniak, 2015

机译：用等温滴定（ITC）和差示扫描量热法（DSC）研究FBP28蛋白片段与铜（II）的络合

Copper(II) complexation by fragment of central part of FBP28 protein from Mus musculus

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