Isolation Methods of Peripheral Blood Mononuclear Cells in Spanish Biobanks: An Overview

Consuegra Irene; Rodriguez-Aierbe Clara; Santiuste Ines; Bosch Anna; Martinez-Marin Rosario; Antonia Fortuto Ma; Diaz Tatiana; Marti Salvador; Angeles Munoz-Fernandez Ma

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Isolation Methods of Peripheral Blood Mononuclear Cells in Spanish Biobanks: An Overview

机译：西班牙语中外周血单核细胞的分离方法：概述

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摘要

The Spanish Hematic Derivatives Group, consisting of 26 biobanks, was established in 2011. We describe here the viability results of our publically available standard operating procedure to freeze and thaw peripheral blood mononuclear cells (PBMCs). Our protocol maximizes PBMC viability while avoiding where possible interbiobank and intrabiobank assay variability.

机译：由26个BioBanks组成的西班牙血液衍生品组成立于2011年。我们在此描述了我们公开的标准操作程序的生存结果，以冻结和解冻外周血单核细胞（PBMC）。我们的协议最大化PBMC活力，同时避免可能的Interbiobank和IntraBiobank测定变异性。

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来源
《Biopreservation and biobanking》 |2017年第4期|共5页
作者
Consuegra Irene; Rodriguez-Aierbe Clara; Santiuste Ines; Bosch Anna; Martinez-Marin Rosario; Antonia Fortuto Ma; Diaz Tatiana; Marti Salvador; Angeles Munoz-Fernandez Ma;
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作者单位

Hosp Gen Univ Gregorio Maranon Spanish HIV HGM BioBank Inst Invest Sanitaria Gregorio Maranon Networking Res Ctr Bioengn Biomat &

Nanomed CIBER Madrid Spain;

Biobanco Vasco Invest OEHUN Vizcaya Spain;

Biobanco Valdecilla Inst Invest Marques de Valdecilla Santander Spain;

IDIBAPS Biobanco Hosp Clin IDIBAPS Inst Invest Biomed August Pi &

Sunyer Barcelona Spain;

BIOBANC MUR Hosp Virgen de la Arrixaca Murcia Spain;

Biobanco Univ Navarra Clin Univ Navarra Pamplona Spain;

Hosp Reg Univ Carlos Haya Malaga Spain;

CIBERER BIOBANK Ctr Invest Biomed Red Enfermedades Raras Valencia Spain;

Hosp Gen Univ Gregorio Maranon Spanish HIV HGM BioBank Inst Invest Sanitaria Gregorio Maranon Networking Res Ctr Bioengn Biomat &

Nanomed CIBER Madrid Spain;

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原文格式 PDF
正文语种 eng
中图分类细胞生物学;
关键词
PBMCs; SOP; biobanks; cryopreservation; functionality;

机译：PBMCS;SOP;BIOBANKS;冷冻保存;功能;

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专利

1. Isolation Methods of Peripheral Blood Mononuclear Cells in Spanish Biobanks: An Overview [J] . Consuegra Irene, Rodriguez-Aierbe Clara, Santiuste Ines, Biopreservation and biobanking . 2017,第4期

机译：西班牙语中外周血单核细胞的分离方法：概述
2. A Modified Ficoll-Paque Gradient Method for Isolating Mononuclear Cells from the Peripheral and Umbilical Cord Blood of Humans for Biobanks and Clinical Laboratories [J] . Jia Yanjuan, Xu Hui, Li Yonghong, Biopreservation and biobanking . 2018,第2期

机译：一种改进的Ficoll-Paque梯度方法，用于将单核细胞分离来自人类的外周和脐脐血，对生物汉和临床实验室
3. Isolation of mononuclear cells of peripheral blood for stem cell transplant: Simplified method [J] . Artaza Sanz Heriberto M, Cortina Rosales Lázaro, Dorticós Balea Elvira, Revista Cubana de Hematología, Inmunología y Hemoterapia . 2008,第3期

机译：用于干细胞移植的外周血单核细胞的分离：简化方法
4. Isolation and Characterization of Interferon-Induced Feline Tetherin (Bst-2) Protein Expressed by Peripheral Blood Mononuclear Cells (PBMCs) [C] . Dyah A. O. A. Pratama, Dyah Kinasih Wuragil, Nia Kurniawan International Conference on Bioinformatics and Nanomedicine from Natural Resources for Biomedical Research . 2019

机译：外周血单核细胞（PBMCs）表达干扰素诱导的猫蛋白（BST-2）蛋白的分离与表征
5. Blood sample processing for the study of aging, and characterization of caspasemRNA expression in peripheral blood mononuclear cells. [D] . Lacelle, Chantale. 2003

机译：血液样品处理，用于研究衰老以及外周血单核细胞中caspasemRNA表达的特征。
6. An Improved Method on Isolation and Serial Passage of Chlamydia pneumoniae From Human Peripheral Blood Mononuclear Cells [O] . Qian Jin, Feihua Huang, Shuming Sun, 2013

机译：一种从人外周血单个核细胞分离和连续传播肺炎衣原体的改进方法
7. Optimization of methods for peripheral blood mononuclear cells isolation and expansion of human gamma delta T cells [O] . Mohd Wajid Ali Khan 2021

机译：优化外周血单核细胞的方法分离和膨胀人γδTT细胞
8. Immune Function in Severe, Active Rheumatoid Arthritis: A Relationship between Peripheral Blood Mononuclear Cell Proliferation to Soluble Antigens and Mononuclear Cell Subset Profiles [R] . Haraoui, B., Wilder, R. L., Malone, D. G., 1984

机译：严重活动性类风湿性关节炎的免疫功能：外周血单核细胞增殖与可溶性抗原和单核细胞亚群谱的关系

Isolation Methods of Peripheral Blood Mononuclear Cells in Spanish Biobanks: An Overview

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