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首页> 外文期刊>Biochemical Engineering Journal >Re-circulative utilization of waste Pichia pastoris as efficient nitrogen source for enhancing butyric acid production
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Re-circulative utilization of waste Pichia pastoris as efficient nitrogen source for enhancing butyric acid production

机译：作为增强丁酸生产的余量利用废磷脂蛋白的循环利用

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摘要

In this study, focusing on efficiently producing butyric acid and utilizing semi-solid waste Pichia pastoris, a novel butyric acid fermentation strategy was developed by using 80 g/L corn as the base medium and the pretreated waste yeast suspension (250 - 400 g/L) as the nitrogen sources. The suspension was added into broth with a volumetric ratio of 1:4 at similar to 20 h, followed by consecutively feeding concentrated glucose solution in 7 L anaerobic bioreactor. With this strategy, butyrate concentration/yield was raised to high levels of 45.0 g/L and 0.40. This strategy could effectively utilize amino acids originated from waste yeast suspension to increase Clostridium tyrobutyricum growth rate/concentration; enhance NADH dependent butyrate synthesis rate under enriched SO42- environment. As a result, butyrate fermentation performance was improved in an indirect way and recirculating bio-waste to replace expensive organic nitrogen sources was realized.
机译：在这项研究中，专注于有效生产丁酸和利用半固体废物磷虾，通过使用80g / l玉米作为基础培养基和预处理的废酵母悬浮液（250-400g /）开发了一种新的丁酸发酵策略 l）作为氮源。 将悬浮液加入肉汤中，其体积比为1：4，与20小时相似，然后在7μlAnaerobic生物反应器中连续进料浓缩葡萄糖溶液。 通过该策略，丁酸浓度/产率升高至45.0g / L和0.40的高水平。 该策略可以有效利用源于废酵母悬浮液的氨基酸，以增加梭菌生长速率/浓度; 增强富含SO42-环境下的NADH依赖性丁酸酯合成率。 结果，以间接方法改善丁酸酯发酵性能，并将生物废物再循环以取代昂贵的有机氮源。

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