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首页> 外文期刊>Cytokine >Production of tumour necrosis factor alpha by primary cultured rat alveolar epithelial cells.
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Production of tumour necrosis factor alpha by primary cultured rat alveolar epithelial cells.

机译：原代培养的大鼠肺泡上皮细胞产生肿瘤坏死因子α。

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Tumour necrosis factor alpha(TNF-alpha) is one of the most important pro-inflammatory cytokines, which plays an important role in host defense and acute inflammation related to tissue injury. The major source of TNF-alpha has been shown to be immune cells such as macrophages and neutrophils. In the present study, we demonstrated that LPS-treatment on alveolar epithelial cells isolated from adult rat lungs also induced a dose- and time-dependent release of TNF-alpha. The purity and identity of these cells were examined by immunofluorescent staining and confocal microscopy with antibodies for cytokeratin and pro-surfactant protein C, markers for epithelial cells and type II pneumocytes respectively. Positive staining of TNF-alpha was observed throughout the cell layer and localized intracellularly. LPS-induced TNF-alpha production from alveolar epithelial cells was blocked not only by cycloheximide, an inhibitor of protein translation, but also by actinomycin D, an inhibitor of gene transcription. The mRNA of TNF-alpha rapidly increased within 1 h of LPS stimulation. These data suggest that LPS-induced TNF-alpha production from alveolar epithelial cells is primarily regulated at the transcriptional level, which is different from that of macrophages and neutrophils. TNF-alpha produced by alveolar epithelial cells may function as an alert signal in host defense to induce production of other inflammatory mediators. Copyright 2000 Academic Press.
机译：肿瘤坏死因子α（TNF-alpha）是最重要的促炎细胞因子之一，在宿主防御和与组织损伤相关的急性炎症中起着重要作用。 TNF-α的主要来源已证明是免疫细胞，例如巨噬细胞和嗜中性粒细胞。在本研究中，我们证明了对从成年大鼠肺中分离出的肺泡上皮细胞进行LPS治疗还诱导了TNF-α的剂量和时间依赖性释放。这些细胞的纯度和特性通过免疫荧光染色和共聚焦显微镜检查，分别用细胞角蛋白抗体和前表面活性剂蛋白C抗体，上皮细胞标记物和II型肺细胞标记物检查。在整个细胞层中观察到TNF-α的阳性染色并定位在细胞内。 LPS诱导的来自肺泡上皮细胞的TNF-α产生不仅被蛋白质翻译抑制剂cycloheximide阻断，而且被基因转录抑制剂放线菌素D阻断。 LPS刺激1小时内，TNF-α的mRNA迅速增加。这些数据表明，LPS诱导的来自肺泡上皮细胞的TNF-α产生主要在转录水平受到调控，这与巨噬细胞和嗜中性粒细胞的转录水平不同。肺泡上皮细胞产生的TNF-α可能在宿主防御系统中充当警报信号，以诱导其他炎症介质的产生。版权所有2000学术出版社。

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