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首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Crystallization of the HigBA2 toxin-antitoxin complex from Vibrio cholerae
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Crystallization of the HigBA2 toxin-antitoxin complex from Vibrio cholerae

机译：霍乱弧菌HigBA2毒素-抗毒素复合物的结晶

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摘要

The genome of Vibrio cholerae encodes two higBA toxin-antitoxin (TA) modules that are activated by amino-acid starvation. Here, the TA complex of the second module, higBA2, as well as the C-terminal domain of the corresponding HigA2 antitoxin, have been purified and crystallized. The HigBA2 complex crystallized in two crystal forms. Crystals of form I belonged to space group P2(1)2(1)2, with unit-cell parameters a = 129.0, b = 119.8, c = 33.4 angstrom, and diffracted to 3.0 angstrom resolution. The asymmetric unit is likely to contain a single complex consisting of two toxin monomers and one antitoxin dimer. The second crystal form crystallized in space group P3(2)21, with unit-cell parameters a = 134.5, c = 55.4 angstrom. These crystals diffracted to 2.2 angstrom resolution and probably contain a complex with a different stoichiometry. Crystals of the C-terminal domain of HigA2 belonged to space group C2, with unit-cell parameters a = 115.4, b = 61.2, c = 73.8 angstrom, beta = 106.7 degrees, and diffracted to 1.8 angstrom resolution.
机译：霍乱弧菌的基因组编码通过氨基酸饥饿激活的两个higBA毒素-抗毒素（TA）模块。在此，第二个模块的复合物higBA2以及相应的HigA2抗毒素的C端结构域已被纯化和结晶。 HigBA2复合物结晶为两种晶体形式。晶型I属于空间群P2（1）2（1）2，单位晶胞参数a = 129.0，b = 119.8，c = 33.4埃，并衍射至3.0埃分辨率。不对称单元可能包含由两种毒素单体和一个抗毒素二聚体组成的单一复合物。在空间群P3（2）21中结晶的第二种晶体形式，其晶胞参数a = 134.5，c = 55.4埃。这些晶体衍射到2.2埃的分辨率，可能包含化学计量不同的复合物。 HigA2 C末端域的晶体属于C2空间群，其晶胞参数a = 115.4，b = 61.2，c = 73.8埃，β= 106.7度，并衍射至1.8埃分辨率。

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