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Inulinase production and mathematical modeling from carob extract by using Aspergillus niger

机译：通过使用Aspergillus尼日尔从Carob提取物中产生和数学建模

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The main objectives of the study were to produce inulinase from carob extract by Aspergillus niger A42 (ATCC 204447) and to model the inulinase fermentation in the optimum carob extract-based medium. In the study, carob extract was used as a novel and renewable carbon source in the production of A. niger inulinase. For medium optimization, eight different variables including initial sugar concentration (degrees Bx), (NH4)(2)HPO4, MgSO4.7H(2)O, KH2PO4, NH4NO3, yeast extract, peptone, and ZnSO4.7H(2)O were employed. After fermentations, optimum medium composition contained 1% yeast extract in 5 degrees Bx carob extract. As a result of the fermentation, the maximum inulinase activity, maximum invertase-type activity, I/S ratio, maximum inulinase- and invertase-type activity rates, maximum sugar consumption rate, and sugar utilization yield were 1507.03 U/ml, 1552.86 U/ml, 0.97, 175.82 and 323.76 U/ml/day, 13.26 g/L/day, and 98.52%, respectively. Regarding mathematical modeling, the actual inulinase production and sugar consumption data were successfully predicted by Baranyi and Cone models based on the model evaluation and validation results and the predicted kinetic values, respectively. Consequently, this was the first report in which carob extract was used in the production of inulinase as a carbon source. Additionally, the best-selected models can serve as universal equations in modeling the inulinase production and sugar consumption in shake flask fermentation with carob extract medium.
机译：该研究的主要目标是通过曲霉A42（ATCC 204447）从CaroB提取物中生产氨基硫酶，并在最佳的Carob提取物的培养基中模拟硫酸脲酶发酵。在该研究中，CaroB提取物被用作尼日尔菊粉酶的生产中的新型和可再生碳源。对于中优化，八种不同的变量，包括初始糖浓度（液体Bx），（NH 4）（2）HPO 4，MgSO4.7H（2）O，KH2PO4，NH 4 NO 3，酵母提取物，蛋白胨和ZnSO4.7H（2）O是雇用。发酵后，最佳培养基组合物在5摄氏度中含有1％酵母提取物。由于发酵，最大菊粉酶活性，最大逆变酶 - 型活性，I / S比，最大菊粉酶和逆变酶型活性率，最高糖耗率和糖利用率为1507.03 U / mL，1552.86 / ml，0.97,175.82和323.76 U / ml /天，13.26克/月/日，分别为98.52％。关于数学建模，基于模型评估和验证结果和预测的动力值，Paranyi和锥形模型成功预测了实际的菊粉酶生产和糖消耗数据。因此，这是第一个报告，其中在菊粉酶作为碳源的生产中使用CAMOB提取物。另外，最佳选择的模型可以用作在用CaroB提取物培养基中施工烧瓶发酵中抑制菊粉酶生产和糖消耗的通用方程。

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