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首页> 外文期刊>Biotechnology Journal: Healthcare,Nutrition,Technology >Using Titer and Titer Normalized to Confluence Are Complementary Strategies for Obtaining Chinese Hamster Ovary Cell Lines with High Volumetric Productivity of Etanercept
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Using Titer and Titer Normalized to Confluence Are Complementary Strategies for Obtaining Chinese Hamster Ovary Cell Lines with High Volumetric Productivity of Etanercept

机译：使用标准化的滴度和磁化是汇率的互补策略，用于获得具有高容量生产率的etanercept的高容量生产力的互补策略

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>The selection of clonally derived Chinese hamster ovary (CHO) cell lines with the highest production rate of recombinant glycoproteins remains a big challenge during early stages of cell line development. Different strategies using either product titer or product titer normalized to cell number are being used to assess suspension‐adapted clones when grown statically in microtiter plates. However, no reported study so far has performed a direct head‐to‐head comparison of these two early reporters for predicting clone performance. Therefore, a screening platform for high‐throughput analysis of titer and confluence of etanercept‐producing clones is developed. Then an unbiased comparison of clone ranking based on either titer or titer normalized to confluence (TTC) is performed. Using two different suspension cultivation vessels, the authors demonstrate that titer‐ or TTC‐based ranking gives rise to the selection of clones with similar volumetric productivity in batch cultures. Therefore, using both titer‐ and TTC‐based ranking is proposed, allowing for selection of distinct clones with both high integral of viable cell density (IVCD) and high specific productivity features, respectively. This contributes to selection of a versatile panel of clones that can be further characterized and from which the final producer clone can be selected that best fits the production requirements.
机译： <第XML：ID =“BIOT201700216-SEC-0001”> >克隆衍生的中国仓鼠卵巢（CHO）细胞系具有重组糖蛋白的最高产量的细胞系仍然是细胞系发育早期阶段的大挑战。使用与细胞数量的产品滴度或产品滴度的不同策略用于评估在微量滴定板静态时悬浮适应的克隆。然而，到目前为止没有报告的研究表达了这两个早期记者的直接对比，以预测克隆性能。因此，开发了一种用于替代替代的螺旋和抵的替代替代替代克隆汇率分析的筛选平台。然后，执行基于标准化为汇合（TTC）的滴度或滴度的克隆排名的无偏偏见比较。使用两种不同的悬浮培养血管，作者证明基于滴度或TTC的排名产生了在分批培养物中选择具有相似体积生产率的克隆。因此，提出了使用基于TTITITS和TTC的排名，允许分别选择具有活性细胞密度（IVCD）和高特异性生产率特征的高积分的不同克隆。这有助于选择可以进一步表征的克隆的多功能面板，并且可以选择最终生产的克隆，以便选择最适合生产要求。

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