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首页> 外文期刊>Cytokine >Effect of 1,25 dihydroxyvitamin D3 on intracellular IFN-gamma and TNF-alpha positive T cell subsets in pulmonary tuberculosis.
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Effect of 1,25 dihydroxyvitamin D3 on intracellular IFN-gamma and TNF-alpha positive T cell subsets in pulmonary tuberculosis.

机译：1,25二羟基维生素D3对肺结核细胞内IFN-γ和TNF-α阳性T细胞亚群的影响。

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摘要

We studied the immunomodulatory effect of 1,25(OH)(2)D(3) on single cell expression of IFN-gamma and TNF-alpha cytokines in T cell subsets of pulmonary tuberculosis (PTB) patients (n=22) and normal healthy subjects (n=22). Peripheral blood mononuclear cells (PBMCs) were cultured with live Mycobacterium tuberculosis (MTB) with or without 1,25(OH)(2)D(3) (10(-7)M) for 48 h. T cell subsets positive for IFN-gamma and TNF-alpha were enumerated by flow cytometry and the culture supernatants were assayed for both the cytokines using ELISA. In both NHS and PTB patients, a significantly reduced percentage of IFN-gamma and TNF-alpha expressing CD3+, CD3+CD4+ and CD3+CD8+ T cells were observed in cultures stimulated with live MTB and treated with 1,25(OH)(2)D(3) compared to cultures without 1,25(OH)(2)D(3) (NHS; CD3+ IFN-gamma+: p<0.0001; CD3+TNF-alpha+: p=0.0292 and PTB; CD3+ IFN-gamma+: p=0.0292; CD3+ TNF-alpha+: p=0.0028). The levels of IFN-gamma and TNF-alpha in the culture supernatants of 1,25(OH)(2)D(3) treated cultures were also found to be significantly decreased in both groups (NHS; IFN-gamma: p=0.0001; TNF-alpha: p<0.0001) and (PTB; IFN-gamma: p<0.0001; TNF-alpha: p<0.0001). A positive correlation was observed between IFN-gamma and TNF-alpha expressing CD3+CD8+ T cells in MTB stimulated cultures treated with or without 1,25(OH)(2)D(3) in NHS (p=0.0001; p=0.001, respectively) and PTB patients (p=0.002; p=0.005, respectively). The present study revealed the suppressive effect of 1,25(OH)(2)D(3) on single cell expression of IFN-gamma and TNF-alpha by CD3+CD4+ and CD3+CD8+ T cells in pulmonary tuberculosis. This suppressive effect of 1,25(OH)(2)D(3) on proinflammatory and Th1 cytokine positive cells might have a role in reducing inflammation at the site of infection.
机译：我们研究了1,25（OH）（2）D（3）对肺结核（PTB）患者（n = 22）和正常人T细胞亚群中IFN-γ和TNF-α细胞因子单细胞表达的免疫调节作用健康受试者（n = 22）。将外周血单核细胞（PBMC）与活结核分枝杆菌（MTB）在有或没有1,25（OH）（2）D（3）（10（-7）M）的情况下培养48小时。通过流式细胞术计数对IFN-γ和TNF-α呈阳性的T细胞亚群，并使用ELISA测定培养上清液中的两种细胞因子。在NHS和PTB患者中，在用活MTB刺激并用1,25（OH）处理的培养物中观察到表达CD3 +，CD3 + CD4 +和CD3 + CD8 + T细胞的IFN-γ和TNF-α的百分比显着降低。 ）D（3）与没有1,25（OH）（2）D（3）的培养物进行比较（NHS; CD3 +IFN-γ+：p <0.0001; CD3 + TNF-alpha +：p = 0.0292和PTB; CD3 +IFN-γ+ ：p ＝ 0.0292; CD3 +TNF-α+：p ＝ 0.0028）。在两组中，经1,25（OH）（2）D（3）处理的培养上清中的IFN-γ和TNF-α水平也均显着降低（NHS;IFN-γ：p = 0.0001 ;TNF-α：p ＜0.0001）和（PTB;IFN-γ：p ＜0.0001;TNF-α：p ＜0.0001）。在经过或未经过NHS的1,25（OH）（2）D（3）处理的MTB刺激培养物中，观察到表达IFN-γ和TNF-α的CD3 + CD8 + T细胞之间呈正相关（p = 0.0001; p = 0.001和PTB患者（分别为p = 0.002，p = 0.005）。本研究揭示了1,25（OH）（2）D（3）对肺结核中CD3 + CD4 +和CD3 + CD8 + T细胞对IFN-γ和TNF-α单细胞表达的抑制作用。 1,25（OH）（2）D（3）对促炎和Th1细胞因子阳性细胞的这种抑制作用可能在减少感染部位的炎症中起作用。

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