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首页> 外文期刊>Cytokine >Up-regulation of IL-33 expression in various types of murine cells by IL-3 and IL-4
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Up-regulation of IL-33 expression in various types of murine cells by IL-3 and IL-4

机译：IL-3和IL-4上调各种类型鼠细胞中IL-33的表达

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The crucial roles of the novel cytokine IL-33 in allergic, inflammatory, infectious and autoimmune diseases are becoming characterized. However, the cytokines which regulate IL-33 expression and secretion are still largely unknown. In this study, IL-3 and IL-4 were found to up-regulate IL-33 mRNA expression in mouse peritoneal exudate cells by a two-color DNA microarray and further confirmed by real time PCR and ELISA. IL-3 and IL-4 synergistically promote IL-33 mRNA expression and IL-33 intracrine in the heterogeneous cell populations as peritoneal exudates cells, bone marrow cells and splenic cells. IL-3 and IL-4 also induced IL-33 introcrine in the peritoneal exudate cells from the macrophage-deficient op/. op mice, suggesting that macrophage is not the only target of IL-3 and IL-4 in the heterogeneous peritoneal exudate cells. Furthermore, IL-3 and IL-4 were verified to promote the IL-33 intracrine in the homogeneous cell population as fibroblasts and mast cells. These results indicate that up-regulation of IL-33 expression by IL-3 and IL-4 is not a feature particular to a specific type of cells. Up to 100 cytokines were screened, but none of them stimulated the secretion or release of IL-33 in the culture system. In summary, we confirm for the first time that IL-3 and IL-4 are critical for IL-33 intracrine in murine cells of various types, indicating that IL-3 and IL-4 may play an important role in the constitutive expression of IL-33 in vivo.
机译：新型细胞因子IL-33在变应性，炎性，感染性和自身免疫性疾病中的关键作用正在得到表征。然而，调节IL-33表达和分泌的细胞因子仍是很大程度上未知的。在这项研究中，发现IL-3和IL-4通过双色DNA微阵列上调小鼠腹膜渗出液细胞中IL-33 mRNA的表达，并通过实时PCR和ELISA进一步证实。 IL-3和IL-4在腹膜渗出液细胞，骨髓细胞和脾细胞中协同促进异种细胞群中IL-33 mRNA表达和IL-33内分泌。 IL-3和IL-4还可以从缺乏巨噬细胞的op /诱导腹膜分泌液中的IL-33内分泌。 op小鼠，表明巨噬细胞不是异种腹膜渗出液细胞中IL-3和IL-4的唯一靶标。此外，已证实IL-3和IL-4可在成纤维细胞和肥大细胞的同质细胞群中促进IL-33内分泌。这些结果表明IL-3和IL-4对IL-33表达的上调不是特定类型细胞特有的特征。筛选了多达100种细胞因子，但它们均未刺激培养系统中IL-33的分泌或释放。总之，我们首次证实IL-3和IL-4对于各种类型的鼠细胞中IL-33内分泌至关重要，这表明IL-3和IL-4可能在IL-3的组成型表达中起重要作用。 IL-33体内。

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