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首页> 外文期刊>Cytokine >Factors influencing the effect of the soluble IL-6 receptor on IL-6 responses in HepG2 hepatocytes.
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Factors influencing the effect of the soluble IL-6 receptor on IL-6 responses in HepG2 hepatocytes.

机译：影响HepG2肝细胞中可溶性IL-6受体对IL-6反应的影响的因素。

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摘要

The soluble IL-6 receptor (sIL-6R) can increase IL-6-induced signalling by forming a complex with IL-6 and membrane-bound gp130 (the receptor beta chain which transduces signals). The conditions affecting this response to sIL-6R were studied using fibrinogen release from HepG2 hepatocytes. Exogenous sIL-6R had no effect alone or in the presence of a submaximal concentration of IL-6, but increased responses to supramaximal IL-6 concentrations in a concentration-related manner. Dexamethasone increased the expression of the membrane IL-6R and endogenous sIL6R release, and increased responses to supramaximal but not submaximal IL-6 concentrations. The amount of endogenous sIL-6R released is relatively small and is unlikely to influence the effects of the exogenous sIL-6R. The observed concentration-related decrease in sIL-6R production in the presence of IL-6 may indicate internalization of ligand/receptor complexes. This would significantly decrease the amount of IL-6R (soluble or membrane) available for signalling and limit continued functional response later in the cultures. These data indicate that the major factor influencing responses to exogenous sIL-6R is an excess of IL-6 which is necessary to form complexes with the sIL-6R, which can then interact with gp130 to increase signalling. Copyright 2000 Academic Press.
机译：可溶性IL-6受体（sIL-6R）可以与IL-6和膜结合的gp130（转导信号的受体β链）形成复合物，从而增加IL-6诱导的信号传导。使用从HepG2肝细胞释放的纤维蛋白原来研究影响此对sIL-6R反应的条件。外源sIL-6R单独或在IL-6浓度未达到最大浓度时均无作用，但以浓度相关的方式增加了对IL-6浓度以上的响应。地塞米松增加了膜IL-6R的表达和内源性sIL6R的释放，并增加了对超最大但非最大IL-6浓度的反应。释放的内源性sIL-6R的量相对较小，不太可能影响外源性sIL-6R的作用。在IL-6存在下观察到的与浓度相关的sIL-6R产量下降可能表明配体/受体复合物内部化。这将显着减少可用于信号传导的IL-6R（可溶性或膜）的量，并限制稍后培养物中的持续功能应答。这些数据表明，影响对外源性sIL-6R反应的主要因素是过量的IL-6，这是与sIL-6R形成复合物所必需的，然后可以与gp130相互作用以增加信号传导。版权所有2000学术出版社。

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