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首页> 外文期刊>Cytokine >Eviprostat suppresses proinflammatory gene expression in the prostate of rats with partial bladder-outlet obstruction: a genome-wide DNA microarray analysis.
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Eviprostat suppresses proinflammatory gene expression in the prostate of rats with partial bladder-outlet obstruction: a genome-wide DNA microarray analysis.

机译：Eviprostat抑制部分膀胱出口阻塞的大鼠前列腺中的促炎基因表达：全基因组DNA微阵列分析。

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摘要

Prostatic inflammation plays a role in the progression of benign prostatic hyperplasia (BPH). Eviprostat is an antioxidant, antiinflammatory phytotherapeutic agent widely used to treat lower urinary tract symptoms in BPH. Because Eviprostat is a mixture of compounds from multiple natural sources, however, its mechanism of action has been difficult to investigate. Here, we describe the use of oligonucleotide microarrays to investigate changes in gene expression in the prostate of rats with surgically induced partial bladder-outlet obstruction and the effect of Eviprostat on those changes. Several dozen proinflammatory genes were activated in obstructed rats, including cytokine, arachidonic acid cascade enzyme, Toll-like receptor (TLR), and transcription factor genes, and their expression was suppressed by Eviprostat. Pathway analysis revealed that several proinflammatory pathways were activated, including cytokine and TLR signaling pathways. The differential expression of selected genes was verified by real-time reverse-transcriptase polymerase chain reaction. Our findings suggest that prostate inflammation in our rat model of partial bladder-outlet obstruction is related to the increased expression of nuclear factor kappaB (NF-kappaB) and the induction of proinflammatory cytokines, and that Eviprostat suppresses their expression at the transcriptional level. The prostate inflammation seen in BPH and the clinical benefits of Eviprostat may be similarly explained.
机译：前列腺炎症在良性前列腺增生（BPH）的进程中起作用。 Eviprostat是一种抗氧化剂，抗炎性植物治疗药，广泛用于治疗BPH中的下尿路症状。由于依维洛司他是多种天然来源的化合物的混合物，因此，其作用机理一直难以研究。在这里，我们描述了使用寡核苷酸微阵列研究具有外科手术诱发的部分膀胱出口梗阻的大鼠前列腺中基因表达的变化以及依维司他对这些变化的影响。在阻塞的大鼠中激活了数十种促炎基因，包括细胞因子，花生四烯酸级联酶，Toll样受体（TLR）和转录因子基因，其表达被Eviprostat抑制。途径分析显示，一些促炎途径被激活，包括细胞因子和TLR信号传导途径。通过实时逆转录酶聚合酶链反应验证了所选基因的差异表达。我们的发现表明，在我们的部分膀胱出口梗阻大鼠模型中，前列腺炎症与核因子κB（NF-κB）的表达增加和促炎性细胞因子的诱导有关，并且依维前列腺素在转录水平上抑制了它们的表达。 BPH所见的前列腺炎症和依维前列腺素的临床益处可以类似地解释。

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