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Membrane fluidization by alcohols inhibits DesK-DesR signalling in Bacillus subtilis

机译:乙醇的膜流化抑制枯草芽孢杆菌的桌面信号传导

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After cold shock, the Bacillus subtilis desaturase Des introduces double bonds into the fatty acids of existing membrane phospholipids. The synthesis of Des is regulated exclusively by the two-component system DesK/DesR; DesK serves as a sensor of the state of the membrane and triggers Des synthesis after a decrease in membrane fluidity. The aim of our work is to investigate the biophysical changes in the membrane that are able to affect the DesK signalling state. Using linear alcohols (ethanol, propanol, butanol, hexanol, octanol) and benzyl alcohol, we were able to suppress Des synthesis after a temperature downshift. The changes in the biophysical properties of the membrane caused by alcohol addition were followed using membrane fluorescent probes and differential scanning calorimetry.
机译:冷休克后,芽孢杆菌枯草芽孢杆菌酶DES将双键引入现有膜磷脂的脂肪酸中。 DES的合成是由双组分系统桌面/ DESR专制的监管; 桌面用作膜状态的传感器,并且在减少膜流动性后触发物合成。 我们的作品的目的是调查能够影响桌子信令状态的膜中的生物物理变化。 使用线性醇(乙醇,丙醇,丁醇,己醇,辛醇)和苄醇,我们能够在温度降档后抑制DES合成。 使用膜荧光探针和差示扫描量热法遵循由酒精添加引起的膜的生物物理性质的变化。

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