• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochimica et biophysica acta. Biomembranes >Cytochrome- c -assisted escape of cardiolipin from a model mitochondrial membrane
【24h】

Cytochrome- c -assisted escape of cardiolipin from a model mitochondrial membrane

机译：来自模型线粒体膜的Carciolipin的细胞色素逃脱

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract Binding of cytochrome c (Cyt c ) to cardiolipin (CL) in the inner mitochondrial membrane is involved with the onset of apoptosis. In this study, we used CL-containing phospholipid monolayers to mimic the inner mitochondrial membrane. Constant pressure insertion assay was employed to monitor the Cyt c -induced expansion of membrane area. Simultaneous epifluorescence microscopy imaging afforded the in-situ visualization of phospholipid demixing and sorting in the membrane. The formation of a CL-rich L d phase has been observed to prelude the insertion of Cyt c . Based on the relative expansion of membrane area, a cluster of a few amino acid residues of Cyt c with an area of 117±7? 2 has been found to insert into the membrane. The insertion of Cytc disrupted the membrane in a way facilitating the escape of CL. When the exclusion of Cyt c was induced by compression, CL molecules appeared to escape the membrane together with the protein, which resulted in a loss of more than a half of CL content from the membrane. These findings may aid in understanding the early events leading to the remodeling of inner mitochondrial membrane and loss of its function during apoptosis. Graphical abstract Display Omitted Highlights ? Penetration capacity of Cyt c for a model mitochondrial membrane is examined. ? ~7 amino acid residues of Cyt c with an area of 117±7 ? 2 insert in the membrane. ? Cyt c -induced demixing and sorting of membrane phospholipids is in situ visualized. ? Cyt c appears to disrupt the membrane in a way facilitating the escape of CL. ? Cyt c -induced modulation in membrane CL content is assessed.
机译：摘要细胞色素C（Cyt C）与内部线粒体膜中的心脂蛋白（Cl）的结合涉及细胞凋亡的发作。在这项研究中，我们使用含Cl的磷脂单层来模拟内部线粒体膜。使用恒压插入测定来监测膜面积的Cyt C-ind膨胀。同时渗流显微镜显微镜成像，其原位可视化的磷脂脱夹和膜中的分选。已经观察到富含CL的L D相的形成术前列Cyt C的插入。基于膜面积的相对膨胀，Cyt C的几个氨基酸残基的簇，面积为117±7？已发现2插入膜中。通过促进Cl逸出的方式，Cytc的插入破坏了膜。当通过压缩诱导Cyt C的排除时，Cl分子似乎与蛋白质一起逸出，导致来自膜的损失超过一半的Cl含量。这些发现可能有助于了解早期事件，导致内部线粒体膜的重塑和在凋亡期间失去其功能。图形抽象显示省略了亮点？研究了模型线粒体膜的Cyt C的渗透能力。还〜7氨基酸残基的Cyt C面积为117±7？ 2插入膜中。还Cyt C-induceded解剖和分选膜磷脂是原位可视化的。还Cyt C似乎以促进Cl逃生的方式扰乱膜。还Cyt C-ind诱导的膜Cl含量的调节。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号