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Transdermal sampling of vitamin D-3 and 25-hydroxyvitamin D-3

机译:维生素D-3和25-羟基维生素D-3的透皮抽样

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Aim: Transdermal analysis is proposed for vitamin D-3 and its hydroxylated metabolite to overcome problems associated with blood analysis. Methods: Vitamin D-3 was extracted directly from skin with solid patches and liquid phases. Deuterium-labeled vitamin D-3 was added to the extraction solutions to compensate for variability and accurately determine the rate of transdermal transfer. Of the different extraction solvents tested, 50:50 octanol:isopropanol showed the best results, with an accuracy of 115% and reproducibility better than 30%. Conclusion: The research shows that transdermal route can be used for analysis of vitamin D-3 in porcine skin. When microneedles are used, accurate measurements were obtained in 1h. With intact skin, the highest accuracy was obtained when extraction was done for 2h.
机译:目的:提出了透皮分析,用于维生素D-3及其羟基化代谢物,以克服与血液分析相关的问题。 方法:将维生素D-3直接从皮肤上用固体贴片和液相萃取。 将氘标记的维生素D-3加入到萃取溶液中以补偿可变性并准确地确定透皮转移速率。 在测试的不同的提取溶剂中,50:50辛醇:异丙醇显示出最佳效果,精度为115%,再现性优于30%。 结论:该研究表明,透皮途径可用于分析猪皮肤中的维生素D-3。 当使用微针时,1小时内得到精确的测量。 皮肤完整,当萃取完成2小时时获得的最高精度。

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