...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cytometry: The Journal of the Society for Analytical Cytology >Monitoring tumour cells in the peripheral blood of small cell lung cancer patients
【24h】

Monitoring tumour cells in the peripheral blood of small cell lung cancer patients

机译：监测小细胞肺癌患者外周血中的肿瘤细胞

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: Flow cytometry was used to enumerate tumour cells in longitudinal studies of peripheral blood from small cell lung cancer (SCLC) patients, together with magnetic bead selection to isolate and identify these cells. As part of a trial, 11 patients received either standard (four weekly) chemotherapy with ifosfamide, carboplatin, and etoposide (ICE) or accelerated (two weekly) ICE with filgrastim (granulocyte colony-stimulating factor [G-CSF]) and autologous stem cell support. Methods: Fresh venous blood was taken throughout treatment and follow-up. Aliquots were stained with a "tumour-specific" antibody against epithelial tissue (Ber EP4), verified as a good marker of SCLC cells by immunohistochemistry. Matched samples labelled with Ber EP4 were separated magnetically by adding a secondary bead-antibody conjugate for confirmation of tumour cell identity. Results: Circulating tumour cells were detected and monitored throughout treatment periods. An initial rise in circulating cells after the first cycle was followed by a fall in both treatment arms to baseline levels set by normal controls. This was achieved by week 12 in the accelerated treatment arm and by week 24 in the standard arm. Conclusions: Flow cytometry and magnetic bead isolation can be used to identify changes in numbers of circulating tumour cells in patients undergoing chemotherapy for SCLC and thereafter during follow-up periods. Absence of tumour cells may indicate a more favourable patient group who would benefit from a more intense course of treatment. (C) 2002 Wiley-Liss, Inc. [References: 27]
机译：背景：在小细胞肺癌（SCLC）患者外周血的纵向研究中，流式细胞仪用于枚举肿瘤细胞，并选择磁珠来分离和鉴定这些细胞。作为试验的一部分，有11例患者接受了标准的（每周四次）联合异环磷酰胺，卡铂和依托泊苷（ICE）的化疗或加速的（两周一次）联合非格司亭（粒细胞集落刺激因子[G-CSF]）和自体干的ICE单元支持。方法：在整个治疗和随访过程中均采集新鲜静脉血。等分试样用针对上皮组织的“肿瘤特异性”抗体（Ber EP4）染色，通过免疫组织化学证实其是SCLC细胞的良好标记。通过添加二级珠-抗体偶联物来磁分离标有Ber EP4的匹配样品，以确认肿瘤细胞的身份。结果：在整个治疗期间检测并监测循环肿瘤细胞。在第一个周期后，循环细胞最初开始上升，随后两个治疗组均下降至正常对照设定的基线水平。这在加速治疗组的第12周和标准组的第24周实现。结论：流式细胞术和磁珠分离可用于鉴定接受SCLC化疗的患者以及随后的随访期间循环肿瘤细胞数量的变化。缺少肿瘤细胞可能表明患者群体更加有利，他们将从更严格的治疗过程中受益。 （C）2002 Wiley-Liss，Inc. [参考：27]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号