...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell and Tissue Research >Expression of neutrophil gelatinase-associated lipocalin (NGAL) in the gut in Crohn's disease
【24h】

Expression of neutrophil gelatinase-associated lipocalin (NGAL) in the gut in Crohn's disease

机译：中性粒细胞明胶酶相关脂素（NGAL）在Crohn病的肠道中的表达

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The antimicrobial glycoprotein neutrophil gelatinase-associated lipocalin (NGAL) is strongly expressed in several infectious, inflammatory and malignant disorders, among these inflammatory bowel disease (IBD). Fecal and serum NGAL is elevated during active IBD and we have recently shown that fecal NGAL is a novel biomarker for IBD with a test performance comparable to the established fecal biomarker calprotectin. This study examines expression of NGAL in the healthy gut and in Crohn's disease (CD), with emphasis on the previously unexplored small intestine. Pinch biopsies were taken from active and inactive CD in jejunum, ileum and colon and from the same sites in healthy controls. Microarray gene expression showed that the NGAL gene, LCN2, was the second most upregulated among 1820 differentially expressed genes in terminal ileum comparing active CD and controls (FC 5.86, p=0.027). Based on immunohistochemistry and in situ hybridization findings, this upregulation most likely represented increased expression in epithelial cells. Double immunofluorescence showed NGAL expression in 49% (range 19-70) of Paneth cells (PCs) in control ileum with no change during inflammation. In healthy jejunum, the NGAL expression in PCs was weak to none but markedly increased during active CD. We further found NGAL also in metaplastic PCs in colon. Finally, we show for the first time that NGAL is expressed in enteroendocrine cells in small intestine as well as in colon.
机译：抗微生物糖蛋白中性粒细胞明胶酶相关的脂素（NGAL）在几种传染病（IBD）中，在几种感染性，炎症和恶性障碍中强烈表达。粪便和血清NGAL在活性IBD期间升高，我们最近显示粪便NGAL是IBD的新型生物标志物，其具有与已建立的粪便生物标志物CALPROTECTIN相当的测试性能。本研究研究了NGAL在健康肠道和克罗恩病（CD）中的表达，重点是以前未开发的小肠。在Jejunum，Hileum和Colon中的活性和无活性CD，以及来自健康对照的同一部位，从活性和无活检中取出。微阵列基因表达表明，NGAL基因LCN2，LCN2在末端对比活性CD和对照中的1820个差异表达基因中的第二个最上调（FC 5.86，P = 0.027）。基于免疫组织化学和原位杂交发现，这种上调最有可能在上皮细胞中表达增加。双免疫荧光显示NGAL表达在49％（范围19-70）的对照对照对照回肠中的49％（PCS）中，在炎症期间没有变化。在健康的Jejunum中，PCS中的NGAL表达在活性CD期间没有显着增加。我们还在结肠的常规PC中进一步发现NGAL。最后，我们首次表明NGAL在小肠中的肠道内分泌细胞和结肠中表达。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号