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首页> 外文期刊>Cell and Tissue Research >Integrin αvβ5 in endothelial cells of rat splenic sinus: An immunohistochemical and ultrastructural study
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Integrin αvβ5 in endothelial cells of rat splenic sinus: An immunohistochemical and ultrastructural study

机译：大鼠脾脏内皮细胞中整合素αvβ5：免疫组化和超微结构研究

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摘要

Localization of integrins β1-8, α1, α2, α3, α5, α6 and αv in sinus endothelial cells of the rat spleen was examined by immunofluorescence microscopy. Labeling for anti-integrin β5 and integrin αv was detected and colocalized in the entire circumference of endothelial cells. Labeling for integrin β5, vinculin and actin filaments demonstrated that they lay close to each other in the basal part of the endothelial cells. Although the other integrin βs, including integrin β1 and integrins α1, α2, α3, α5 and α6 in combination with integrin β1, were localized in leukocytes, slightly large cells, megakaryocytes and/or platelets in the sinus lumen and splenic cords, they were not detected in endothelial cells. Labeling for vitronectin, a component of the extracellular-matrix-binding integrin αvβ5, was strongly stained in the periphery of the wall of sinuses, as was collagen IV and, in addition, was localized in the cytoplasm of endothelial cells. Ultrastructural localization of integrin β5, vitronectin and clathrin was examined by immunogold electron microscopy to elucidate the involvement of integrin αvβ5 in the endocytosis of vitronectin in sinus endothelial cells. Electron microscopy with detergent extraction revealed abundant coated pits and coated vesicles in endothelial cells. Immunogold labeling for vitronectin was present in pits, vesicles and the stacked endoplasmic reticulum. Double-labeling for integrin β5 or integrin αv and clathrin revealed that they were colocalized in some vesicles in close proximity to the apical and lateral plasma membrane of the endothelial cells. The possible functional roles of integrin αvβ5 in endothelial cells of the splenic sinus are discussed.
机译：通过免疫荧光显微镜检查鼻窦内皮细胞中整合β1-8，α1，α2，α3，α5，α6和αv的定位。检测抗整合蛋白β5和整合蛋白αv的标记，并在内皮细胞的整个圆周中分开。对整联蛋白β5的标记，Vinculin和肌动蛋白细丝表明它们在内皮细胞的基部部分彼此静置。虽然其他整联蛋白βS，包括整合素β1和整合α1，α2，α3，α5和α6与整合蛋白β1组合的，但在白细胞，略微大的细胞，巨大的细胞，甲状腺细胞和/或血小板中局部化，它们是未在内皮细胞中检测到。 vitronectin的标记，细胞外基质结合整合蛋白αvβ5的组分在鼻窦的周边中染色，如胶原素IV，并且另外，局部化在内皮细胞的细胞质中。通过免疫电解电子显微镜检查整合蛋白β5，VITRONECTIN和Clathrin的超微结构定位，阐明整联蛋白αvβ5在窦内皮细胞中葡ITronectin的内吞作用中的累积。具有洗涤剂萃取的电子显微镜显微镜在内皮细胞中显示丰富的涂层凹坑和涂层囊泡。 VITRONECTIN的免疫形状标记存在于凹坑，囊泡和堆叠的内质网中。对整联蛋白β5或整合素αv和clathrin的双标记显示，它们在内皮细胞的顶端和横向质膜附近的一些囊泡中被覆盖。讨论了整合蛋白αvβ5在脾窦的内皮细胞中的可能官能团。

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