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首页> 外文期刊>Cell and Tissue Research >Distinct differences between cultured podocytes and parietal epithelial cells of the Bowman's capsule
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Distinct differences between cultured podocytes and parietal epithelial cells of the Bowman's capsule

机译：鲍曼胶囊的培养泛细胞和垂直上皮细胞之间的明显差异

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摘要

Phenotypic changes in culture hamper the identification and characterization of cultured podocytes and parietal epithelial cells of the Bowman's capsule (PECs). We have recently established culture conditions that restore podocytes to their differentiated phenotypes. We compared podocytes and PECs cultured under the same conditions to determine the unique characteristics of the two cell types. Performing this comparison under the same conditions accentuated these differences. Podocytes behaved like non-epithelial cells by extending cell processes even at confluence. By contrast, PECs behaved like typical epithelial cells by maintaining a polygonal appearance. Other differences were identified using immunostaining and RT-PCR; podocytes expressed high levels of podocyte-specific markers while PECs expressed high levels of PEC-specific markers. However, while podocytes expressed low levels of PEC markers, PECs expressed low levels of podocyte markers. Therefore, the identification of podocytes and PECs in culture requires the evaluation of respective cell markers and the expression of markers for other cell types.
机译：文化的表型变化阻碍了鲍曼胶囊（PECS）培养的诱惑诱导诱惑的诱导诱导的诱饵和垂直上皮细胞。我们最近建立了恢复瘘管的培养条件，以其分化的表型。我们比较了在相同条件下培养的诱饵和PEC，以确定两种细胞类型的独特特征。在相同的条件下执行此比较，突出了这些差异。甚至在汇合下延伸细胞过程，达粒细胞表现为非上皮细胞。相比之下，通过维持多边形外观，PECS表现得像典型的上皮细胞。使用免疫染色和RT-PCR鉴定其他差异;大织物表达了高水平的podyte特异性标记，而PEC表达了高水平的PEC特异性标记。然而，虽然大织物表达了低水平的PEC标记，但PEC表达了低水平的孔细胞标记物。因此，培养的诱导诱导诱导培养物和PECs需要评估各种细胞标志物和用于其他细胞类型的标志物的表达。

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