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首页> 外文期刊>Cell cycle >Up-regulated lncRNA GAS5 promotes chemosensitivity and apoptosis of triple-negative breast cancer cells
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Up-regulated lncRNA GAS5 promotes chemosensitivity and apoptosis of triple-negative breast cancer cells

机译：上调的LNCRNA气体5促进三阴性乳腺癌细胞的化学敏感性和凋亡

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摘要

Up to accomplishment of this study, the role of long non-coding RNAs (lncRNAs) in breast cancer has been investigated in several researches. Nevertheless, its association with the chemosensitivity of cancer was little known. Therefore, this study is focused on lncRNA GAS5 and its influence in the chemosensitivity of triple-negative breast cancer (TNBC). Expression of GAS5 in TNBC tissues and cells was detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR) and its methylation was evaluated using methylation-specific polymerase chain reaction (MSP). Moreover, in order to define the contributory role of GAS5 in TNBC, GAS5 expression, proliferation, and apoptosis of TNBC cells were detected by a series of experiment. Finally, the effects of GAS5 in vivo were investigated by measuring tumor formation in nude mice. GAS5 was poorly expressed in TNBC tissues and cells, which could regulate the progression of TNBC. The methylation of CpG island in the promoter region of GAS5 in MDA-MB-231 and MDA-MB-468 cells was decreased, while GAS5 expression in cells was increased. Overexpressed GAS5 reduced the inhibitory concentration (IC50) value and the cell proliferation of TNBC, and promoted their apoptosis, so as to delay the progression of TNBC. Our study provides evidence that up-regulated GAS5 suppressed the progression of TNBC and promoted chemosensitivity and apoptosis of TNBC cells. Thus, GAS5 may be a potential candidate for the treatment of TNBC.
机译：达到本研究的完成，在几项研究中已经研究了长期非编码RNA（LNCRNA）在乳腺癌中的作用。尽管如此，它与癌症的化学敏感度的关联很少。因此，该研究专注于LNCRNA气体5及其对三阴性乳腺癌（TNBC）的化学敏感性的影响。通过逆转录量相分聚物链反应（RT-QPCR）检测TNBC组织和细胞中的Gas5在TNBC组织和细胞中的表达，并使用甲基化特异性聚合酶链式反应（MSP）评价其甲基化。此外，为了定义Gas5在TNBC中的贡献作用，通过一系列实验检测到TNBC细胞的Gas5表达，增殖和凋亡。最后，通过测量裸鼠中的肿瘤形成来研究气体体5在体内的影响。 Gas5在TNBC组织和细胞中表达不当，其可以调节TNBC的进展。在MDA-MB-231和MDA-MB-468细胞中，Gas5启动子区域中CpG岛的甲基化，同时增加了细胞中的气体5表达。过表达气体5降低了TNBC的抑制浓度（IC 50）值和细胞增殖，并促进其凋亡，从而延迟TNBC的进展。我们的研究提供了证据表明上调的Gas5抑制了TNBC的进展并促进了TNBC细胞的化学敏感性和凋亡。因此，Gas5可以是用于治疗TNBC的潜在候选者。

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  • 来源
    《Cell cycle》 |2019年第16期|共11页
  • 作者

    Li Juntao; Li Lin; Yuan Huozhong; Huang Xing-Wei; Xiang Tianxin; Dai Sujuan;

  • 作者单位

    Peoples Hosp Ganzhou City Dept Breast &

    Thyroid Surg Ganzhou Jiangxi Peoples R China;

    Peoples Hosp Ganzhou City Dept Pharm Intravenous Admixture Serv Ganzhou Jiangxi Peoples R China;

    Peoples Hosp Ganzhou City Dept Breast &

    Thyroid Surg Ganzhou Jiangxi Peoples R China;

    Peoples Hosp Ganzhou City Dept Breast &

    Thyroid Surg Ganzhou Jiangxi Peoples R China;

    Nanchang Univ Affiliated Hosp 1 Dept Infect Dis Nanchang Jiangxi Peoples R China;

    Peoples Hosp Ganzhou City Dept Pathol Ganzhou Jiangxi Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    GAS5; methylation; triple-negative breast cancer;

    机译：Gas5;甲基化;三阴性乳腺癌;

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