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首页> 外文期刊>Cell cycle >The long non-coding RNA CRNDE competed endogenously with miR-205 to promote proliferation and metastasis of melanoma cells by targeting CCL18
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The long non-coding RNA CRNDE competed endogenously with miR-205 to promote proliferation and metastasis of melanoma cells by targeting CCL18

机译：长期非编码RNA CRNDE与miR-205竞争，通过靶向CCL18促进黑素瘤细胞的增殖和转移

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摘要

Melanoma was the most malignant skin neoplasm with an increasing morbidity around the world. Although new immunotherapies and targeted therapies have emerged recently, the long-term survival of melanoma patients still remains low. To reveal effective diagnostic methods and therapeutic strategies, the potential mechanism of melanoma is urgently needed to be studied. Long non-coding RNAs (lncRNAs) have become an important regulatory factor in the occurrence and development of cancer, and it can be used as a new prognostic and diagnostic marker. In this study, we aimed to inspect the effects of lncRNA colorectal neoplasia differentially expressed (CRNDE) on the melanoma cell viability, invasion and migration. After microarray analysis, 106 dysregulated lncRNAs and 1187 abnormally expressed mRNAs were screened out. Further, the lncRNA CRNDE and CCL18 expression in melanoma tissues and cell lines were examined. It was determined that they were both overexpressed in melanoma tissues and cell lines. The down-regulation of lncRNA CRNDE and CCL18 induced melanoma cell apoptosis and inhibited cell viability. Then, miR-205 which had binding site with lncRNA CRNDE and CCL18 was involved in the next experiment, and it was down-regulated in melanoma that negatively correlated with lncRNA CRNDE expression. In addition, overexpression of miR-205 results in the restore of cell viability and aggressiveness. In conclusion, LncRNA CRNDE promotes the migration and invasion of melanoma by sponging miR-205 and releasing CCL18.
机译：黑色素瘤是最恶毒的皮肤肿瘤，世界各地的发病率越来越大。虽然最近出现了新的免疫治疗和有针对性的疗法，但黑色素瘤患者的长期存活仍然仍然低。为了揭示有效的诊断方法和治疗策略，迫切需要研究黑素瘤的潜在机制。长期非编码RNA（LNCRNA）已成为癌症发生和发展的重要调节因素，可用作新的预后和诊断标志物。在这项研究中，我们旨在检查Lncra Na结直肠瘤形成差异表达（CRNDE）对黑色素瘤细胞活力，侵袭和迁移的影响。在微阵列分析后，筛选出异常表达MRNAs的106个疑虑的LNCRNA和1187。此外，检查了黑色素瘤组织和细胞系中的LNCRNA CRNDE和CCL18表达。确定它们在黑素瘤组织和细胞系中均过表达。 LNCRNA CRNDE和CCL18诱导的黑素瘤细胞凋亡的下调和抑制细胞活力。然后，在下一个实验中涉及具有LNCRNA CRNDE和CCL18的结合位点的miR-205，并在黑色素瘤中抑制与LNCRNA CRNDE表达呈负相关的黑色素瘤。此外，miR-205的过表达导致恢复细胞活力和侵略性。总之，LNCRNA CRNDE通过海绵MIR-205促进黑素瘤的迁移和侵袭并释放CCL18。

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  • 来源
    《Cell cycle》 |2018年第18期|共13页
  • 作者

    Xu Libin; Zhang Yu; Zhao Zhenguo; Chen Zhengju; Wang Zheng; Xu Songfeng; Zhang Xinxin; Liu Ting; Yu Shengji;

  • 作者单位

    Chinese Acad Med Sci Canc Hosp Natl Clin Res Ctr Canc Natl Canc Ctr Dept Orthoped Surg Beijing;

    Peking Union Med Coll Beijing Peoples R China;

    Chinese Acad Med Sci Canc Hosp Natl Clin Res Ctr Canc Natl Canc Ctr Dept Orthoped Surg Beijing;

    Beijing 100biotech Co Ltd Technol Ctr Tech Consultant Dept Beijing Peoples R China;

    Peking Union Med Coll Beijing Peoples R China;

    Chinese Acad Med Sci Canc Hosp Natl Clin Res Ctr Canc Natl Canc Ctr Dept Orthoped Surg Beijing;

    Chinese Acad Med Sci Canc Hosp Natl Clin Res Ctr Canc Natl Canc Ctr Dept Orthoped Surg Beijing;

    Chinese Acad Med Sci Canc Hosp Natl Clin Res Ctr Canc Natl Canc Ctr Dept Orthoped Surg Beijing;

    Chinese Acad Med Sci Canc Hosp Natl Clin Res Ctr Canc Natl Canc Ctr Dept Orthoped Surg Beijing;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    Mir-205; CCL18; lncRNA CRNDE; melanoma;

    机译：mir-205;ccl18;lncrna crnde;黑色素瘤;

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