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首页> 外文期刊>Chemical and Biochemical Engineering Quarterly >Production of D-Glyceric Acid by a Two-step Culture Strategy Based on Whole-cell Biocatalysis of Acetobacter tropicalis
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Production of D-Glyceric Acid by a Two-step Culture Strategy Based on Whole-cell Biocatalysis of Acetobacter tropicalis

机译：基于全细胞生物分析的双步培养策略对乙酰曲杆菌的双层培养策略产生D-甘油酸

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摘要

D-Glyceric acid (D-GA) is a promising chemical for minimizing the toxic effect of acetaldehyde. It has been successfully produced by bioprocesses using glycerol as a substrate. However, high concentrations of glycerol are not beneficial for cell growth. A two-step culture strategy was employed to deal with the ambivalent culture conditions between the growth of Acetobacter tropicalis and the biosynthesis of D-GA. The first stage focuses on biomass accumulation with low initial glycerol concentration; the second stage provides D-GA accumulation through whole-cell biocatalysis. Approximately 2-fold of D-GA yield and 4.5-fold of D-GA productivity were gained by the two-step culture strategy compared to the traditional fermentation with 150 g L-1 of glycerol. Our results also showed that A. tropicalis was able to catalyze the conversion of glycerol to D-GA in the presence of up to 10 % methanol. These findings have important implications to enhance D-GA yield by strategy optimization and reduce its cost.
机译：D-甘甘油（D-GA）是最有希望的化学品，可最大限度地减少乙醛的毒性作用。它已经通过使用甘油作为基材的生物处理成功生产。然而，高浓度的甘油对于细胞生长并不有益。采用两步文化策略来处理乙酰曲杆菌的生长和D-GA的生物合成之间的矛盾培养条件。第一阶段侧重于具有低初始甘油浓度的生物质积累;第二阶段通过全细胞生物分析提供D-GA积累。与具有150g L-1的甘油的传统发酵相比，两步培养策略获得了大约2倍的D-GA产率和45倍的D-GA生产率。我们的研究结果还表明，A.Tropicalis能够在高达10％甲醇存在下催化甘油转化为D-Ga。这些调查结果具有重要意义，以通过策略优化提高D-GA产量并降低其成本。

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