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Lipid Bilayer Membrane of Egg-PC Liposome Evaluated as Chemical Reaction Field with Picosecond Time-Resolved Fluorescence Spectroscopy

机译:蛋绒脂质体的脂质双层膜作为化学反应场与PICOSECOND时间分离的荧光光谱

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摘要

Viscosity inside the lipid bilayer of egg-PC (egg yolk phosphatidylcholine) liposome with a diameter of 100 nm is examined with picosecond time-resolved fluorescence spectroscopy. The viscosity is estimated independently from the photoisomerization rate and from the rotational relaxation time of the first excited singlet state of trans-stilbene solubilized within the lipid bilayer. The presence of two solvation environments within the bilayer is suggested from both of the methods. One environment is 50 to 100 times more viscous than the other. The use of trans-stilbene as a probe provides valuable information on the solvation environments inside the lipid bilayer of liposome, which serves as a field for a number of biochemical reactions.
机译:用Piposecond时间分辨荧光光谱检查具有直径为100nm的蛋绒(蛋黄磷脂酰胆碱)脂质体内的脂质双层脂质双层的粘度。 粘度从光学异构率和脂质双层内溶解在脂质双层内溶解的第一激发态单态的旋转弛豫时间估计粘度。 两种方法都提出了双层内两种溶剂化环境的存在。 一个环境比另一个环境粘稠50到100倍。 作为探针的使用反式 - 斯蒂莱烯提供了有关脂质体脂质化体内脂质化合物内的溶剂化环境的有价值的信息,其用作许多生物化学反应的田间。

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