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首页> 外文期刊>Bulletin of the Chemical Society of Japan >A Triple-Electrode Based Dual-Biosensor System Utilizing Track-Etched Microporous Membrane Electrodes for the Simultaneous Determination of L-Lactate and D-Glucose
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A Triple-Electrode Based Dual-Biosensor System Utilizing Track-Etched Microporous Membrane Electrodes for the Simultaneous Determination of L-Lactate and D-Glucose

机译：用于同时测定L-乳酸和D-葡萄糖的轨道蚀刻微孔膜电极的三电极基双生物传感器系统

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摘要

A new triple-electrode system was developed for the simultaneous determination of L-lactate and D-glucose by using track-etched microporous membrane electrodes, which enabled efficient electrolysis as electroactive substances passed through the electrode. The proposed biosensor was fabricated by alternate stacking of the electrodes and immobilized enzyme reactors along the flow direction of the sample solution. When the sample solution containing L-lactate and D-glucose flowed into the proposed biosensor, hydrogen peroxide was produced by each enzymatic reaction and detected individually by anodization at each detector-electrode positioned downstream of each enzyme reactor. The interfering substances, such as L-ascorbic acid and uric acid, were electrolyzed completely at the prereactor electrode that was positioned upstream of the enzyme reactors. As a result, the simultaneous detection of L-lactate and D-glucose was achieved with no other catalytic material such as peroxidase or an electron mediator in the presence of the interferents at physiological concentrations in human blood.
机译：开发了一种新的三电极系统，用于通过使用轨道蚀刻的微孔膜电极来同时测定L-乳酸和D-葡萄糖，这使得能够有效地电解为通过电极的电活性物质。通过沿着样品溶液的流动方向交替堆叠电极和固定化的酶反应器来制造所提出的生物传感器。当含有L-乳酸的样品溶液和流入所提出的生物传感器的D-葡萄糖时，通过每个酶促反应产生过氧化氢，并通过在每个酶反应器的下游的每个检测器电极下通过阳极氧化单独检测。干扰物质，例如L-抗坏血酸和尿酸，完全电解在位于酶反应器的上游的prerector电极。结果，在存在于人体血液中的生理浓度的干扰物存在下，没有其他催化材料如过氧化物酶或电子介质的同时检测L-乳酸盐和D-葡萄糖。

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    Mizuguchi Hitoshi; Sasaki Keiko; Ichinose Hirokazu; Seino Shota; Sakurai Jun; Iiyama Masamitsu; Kijima Tatsuro; Tachibana Kazuhiro; Nishina Tatsuo; Takayanagi Toshio; Shida Junichi;

  • 作者单位

    Tokushima Univ Grad Sch Sci &

    Technol Dept Appl Chem Tokushima 7708506 Japan;

    Yamagata Univ Grad Sch Sci &

    Engn Yonezawa Yamagata 9928510 Japan;

    Yamagata Univ Grad Sch Sci &

    Engn Yonezawa Yamagata 9928510 Japan;

    Yamagata Univ Grad Sch Sci &

    Engn Yonezawa Yamagata 9928510 Japan;

    Yamagata Univ Grad Sch Sci &

    Engn Yonezawa Yamagata 9928510 Japan;

    Nomura Micro Sci Co Ltd 2-4-37 Okada Atsugi Kanagawa 2430021 Japan;

    Yamagata Univ Grad Sch Sci &

    Engn Yonezawa Yamagata 9928510 Japan;

    Yamagata Univ Grad Sch Sci &

    Engn Yonezawa Yamagata 9928510 Japan;

    Yamagata Univ Grad Sch Sci &

    Engn Yonezawa Yamagata 9928510 Japan;

    Tokushima Univ Grad Sch Sci &

    Technol Dept Appl Chem Tokushima 7708506 Japan;

    Yamagata Univ Grad Sch Sci &

    Engn Yonezawa Yamagata 9928510 Japan;

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  • 正文语种 eng
  • 中图分类 化学;
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