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首页> 外文期刊>Carbohydrate research >Development and application of a HPLC-MS/MS method for quantitation of fucosylated chondroitin sulfate and fucoidan in sea cucumbers
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Development and application of a HPLC-MS/MS method for quantitation of fucosylated chondroitin sulfate and fucoidan in sea cucumbers

机译：HPLC-MS / MS法定量岩石化软骨素硫酸盐和岩藻糖蛋白硫酸盐素硫酸盐和岩藻糖膜的研制及应用

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摘要

Fucosylated chondroitin sulfate (FCS) and fucoidan (FUC) are two main bioactive polysaccharides in sea cucumbers. A novel method for quantitation of FCS and FUC was developed by detecting chondroitin disaccharide and fucose produced through acid hydrolysis using HPLC-MS/MS. The present method showed satisfactory performance for both saccharides. It was applied to assay sea cucumbers (Stichopus japonicus) reared in pond grow-out or bottom sowing, and the results were compared with those obtained by traditional HPLC method and 1,9-dimethylmethylene blue test, which could only provide the total sea cucumber polysaccharide (SCP) contents. No difference of total SCP content was observed between sea cucumbers reared through different ways, while a higher ratio of FCS to FUC of sea cucumbers of pond grow-out was revealed by the present method. Thus, this novel method is potential to quantify the two polysaccharides and could be a powerful tool for quality evaluation of sea cucumbers.
机译：岩石化的软骨素（FCS）和FCOONOINAN（FUC）是海参中的两种主要生物活性多糖。 通过使用HPLC-MS / MS检测通过酸性水解的软骨素二糖和岩藻糖来制定一种用于定量FCS和FUC的新方法。 本方法表明两种糖类的令人满意的性能。 它被应用于在池塘成长或底部播种的测定海参（Stichopus japonicus），并将结果与通过传统HPLC方法获得的结果和1,9-二甲基亚甲基蓝试验进行了比较，这只能提供总海参 多糖（SCP）内容物。 在通过不同方式饲养的海参之间没有观察到总SCP含量的差异，而目前方法揭示了池塘中池塘中的海洋黄瓜的FCS与海参的较高比率。 因此，这种新方法是量化两种多糖的潜力，并且可能是海参质量评估的强大工具。

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  • 来源
    《Carbohydrate research》 |2018年第2018期|共7页
  • 作者

    Zhenjun Zhu; Beiwei Zhu; Chunqing Ai; Jiaojiao Lu; Sufeng Wu; Yili Liu; Linlin Wang; Jingfeng Yang; Shuang Song; Xiaoling Liu;

  • 作者单位

    College of Light Industry and Food Engineering Guangxi University;

    College of Light Industry and Food Engineering Guangxi University;

    School of Food Science and Technology National Engineering Research Center of Seafood Dalian Polytechnic University;

    School of Food Science and Technology National Engineering Research Center of Seafood Dalian Polytechnic University;

    School of Food Science and Technology National Engineering Research Center of Seafood Dalian Polytechnic University;

    School of Food Science and Technology National Engineering Research Center of Seafood Dalian Polytechnic University;

    School of Food Science and Technology National Engineering Research Center of Seafood Dalian Polytechnic University;

    School of Food Science and Technology National Engineering Research Center of Seafood Dalian Polytechnic University;

    School of Food Science and Technology National Engineering Research Center of Seafood Dalian Polytechnic University;

    College of Light Industry and Food Engineering Guangxi University;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 有机化学;
  • 关键词

    Sea cucumber polysaccharides; Fucosylated chondroitin sulfate; Fucoidan; Acid hydrolysis; HPLC–MS/MS;

    机译：海参多糖;硫酸岩石化的软骨素硫酸盐;岩藻酮;酸水解;HPLC-MS / MS;

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