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首页> 外文期刊>Acta Histochemica: Zeitschrift fur Histologische Topochemie >Histophysiological effects of fluid resuscitation on heart, lung and brain tissues in rats with hypovolemia.
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Histophysiological effects of fluid resuscitation on heart, lung and brain tissues in rats with hypovolemia.

机译：液体复苏对血容量不足大鼠心，肺和脑组织的组织生理学影响。

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The efficacy of using colloids and crystalloids in the treatment of hypovolemia still remains controversial. An important aspect in treating hypovolemia is to re-establish normal tissue hemodynamics after fluid resuscitation. Production of nitric oxide (NO) or growth factors such as transforming growth factor beta (TGF-beta) has been identified as a key mechanism in physiological and pathological processes in the different systems. This study was designed to investigate the histophysiological effects of resuscitation with different plasma substitutes on the heart, lung and brain tissues following acute blood loss in male Sprague-Dawley rats weighing 250-280g (n=30). After anesthesia with sodium pentobarbital, the left femoral vein and artery were cannulated for the administration of volume expanders and for direct measurement of arterial pressure and heart rate. Twenty rats were bled (5ml/10min) and infused (5ml/10min) with one of four randomly selected solutions, (a) human albumin, (b) gelatin (Gelofusine), (c) dextran-70 (Macrodex); or (d) physiological saline (0.9% isotonic saline). Five control rats were bled without infusion. Tissue samples were taken and fixed in 10% formalin solution, then processed for embedding in paraffin wax. Sections were cut and stained with hematoxylin and eosin. Indirect immunohistochemical labelling was performed to reveal binding of primary antibodies against endothelial nitric oxide synthase (eNOS), inducible nitric oxide synthase (iNOS) and TGF-beta. Mild immunoreactivity of eNOS was observed in endothelial cells of vessels in brain, heart and lung tissues. Increased immunoreactivities of eNOS, iNOS and TGF-beta were observed in the non-fluid resuscitated group in these organs; mild, moderate, moderate and strong immunoreactivities were seen in the albumin, gelatin, physiological saline and dextran-70 treated groups, respectively. Immunoreactivities of iNOS and TGF-beta in the non-fluid resuscitated group were increased significantly, in comparison to the other groups, apart from the dextran-70 treated group. The results of this study show that gelatin solution and physiological saline may be of use after acute blood loss, and dextran-70 is not the preferred resuscitation fluid in the early stages of acute blood loss. It was concluded that albumin solution is the preferred fluid for resuscitation.
机译：使用胶体和晶体治疗血容量不足的疗效仍存在争议。治疗血容量不足的一个重要方面是在液体复苏后重新建立正常的组织血流动力学。一氧化氮（NO）或生长因子（例如转化生长因子β（TGF-beta））的产生已被确定为不同系统中生理和病理过程的关键机制。这项研究旨在调查在体重250-280g（n = 30）的雄性Sprague-Dawley大鼠急性失血后使用不同血浆替代品进行复苏对心脏，肺和脑组织的组织生理学影响。用戊巴比妥钠麻醉后，对左股静脉和动脉进行插管，以进行体积扩展器的使用并直接测量动脉压和心率。给20只大鼠放血（5ml / 10min），并输注（5ml / 10min）四种随机选择的溶液之一，（a）人白蛋白，（b）明胶（Gelofusine），（c）葡聚糖-70（Macrodex）;或（d）生理盐水（0.9％等渗盐水）。五只对照大鼠不输血而放血。采集组织样品并固定在10％福尔马林溶液中，然后进行处理以包埋在石蜡中。切下切片并用苏木精和曙红染色。进行了间接免疫组化标记，以揭示针对内皮一氧化氮合酶（eNOS），诱导型一氧化氮合酶（iNOS）和TGF-β的一抗的结合。在脑，心脏和肺组织的血管内皮细胞中观察到了轻度的eNOS免疫反应性。在这些器官的非流体复苏组中，观察到eNOS，iNOS和TGF-β的免疫反应性增加。在白蛋白，明胶，生理盐水和右旋糖酐70处理组中分别观察到轻，中，中和强免疫反应。与其他组相比，除右旋糖酐70治疗组外，非流体复苏组中iNOS和TGF-β的免疫反应性显着增加。这项研究的结果表明，急性失血后可以使用明胶溶液和生理盐水，而在急性失血的早期阶段，右旋糖酐70并不是首选的复苏液。结论是白蛋白溶液是复苏的首选液体。

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