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首页> 外文期刊>Acta Horticulturae >In vitro behaviour of different hosts infected with different fruit tree mycoplasma-like organisms
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In vitro behaviour of different hosts infected with different fruit tree mycoplasma-like organisms

机译：不同宿主感染不同果树支原体样生物的体外行为

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摘要

Since 1985, a collection of non-culturable phytoplasmas infecting various fruit trees has been maintained in their natural and experimental hosts propagated in vitro. Different isolates of Malus-affecting phytoplasmas associated with apple proliferation were multiplied in M. pumila rootstock MM106 and in the woody indicator Pyronia veitchii. Different isolates of Prunus-affecting phytoplasmas associated with severe diseases like apricot chlorotic leaf roll, Molieres disease and plum decline, were maintained in the Prunus marianna rootstock GF 8-1 or in the Prunus domestica cv. Enduke Tardicotes. Two fruit tree phytoplasma isolates, which had been experimentally transmitted by dodder to Catharanthus roseus, were also studied. Phytoplasma-infected shoot tips of C. roseus were cultured in vitro. PCR amplification of phytoplasma-specific DNA fragments were used to confirm the presence of different phytoplasma isolates in infected micropropagated plants. DAPI staining was used to estimate the phytoplasma concn in plants. The in vitro behaviour of different host plants infected with various phytoplasma isolates was studied by analysing growth parameters, symptom expression and phytoplasma concn.
机译：自1985年以来，感染了各种果树的不可培养的植物质原体一直保存在其自然的和体外繁殖的实验宿主中。与苹果增生相关的影响苹果的苹果醇溶性质原体的不同分离株在穆氏支原体根茎MM106和木质指示剂Pyronia veitchii中繁殖。与严重疾病如杏黄绿叶卷叶病，莫里莱斯病和李子衰弱有关的影响李的植物原质的不同分离物被保存在玛丽李砧木GF 8-1或李树中。 Enduke Tardicotes。还研究了两种果树分离株，这些分离株已通过do丝剂实验性地传播到了长春花。体外培养了被质体感染的玫瑰假丝酵母的茎尖。植物质体特异性DNA片段的PCR扩增用于确认感染的微繁殖植物中存在不同的植物质分离株。 DAPI染色用于估计植物中的植物质体浓度。通过分析生长参数，症状表达和植物浆体浓度，研究了感染了各种植物浆体分离株的不同寄主植物的体外行为。

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