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首页> 外文期刊>Acta microbiologica et immunologica Hungarica: A quarterly of the Hungarian Academy of Sciences >Characterisation of community structure of bacteria in parallel mesophilic and thermophilic pilot scale anaerobe sludge digesters.
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Characterisation of community structure of bacteria in parallel mesophilic and thermophilic pilot scale anaerobe sludge digesters.

机译：中温和嗜热中试厌氧污泥消化池中细菌群落结构的表征。

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摘要

The aim of the present work was to compare the microbial communities of a mesophilic and a thermophilic pilot scale anaerobe sludge digester. For studying the communities cultivation independent chemotaxonomical methods (RQ and PLFA analyses) and T-RFLP were applied. Microbial communities of the mesophilic and thermophilic pilot digesters showed considerable differences, both concerning the species present, and their abundance. A Methanosarcina sp. dominated the thermophilic, while a Methanosaeta sp. the mesophilic digester among Archaea. Species diversity of Bacteria was reduced in the thermophilic digester. Based on the quinone patterns in both digesters the dominance of sulphate reducing respiratory bacteria could be detected. The PLFA profiles of the digester communities were similar though in minor components characteristic differences were shown. Level of branched chain fatty acids is slightly lower in the thermophilic digester that reports less Gram positive bacteria. The relative ratio of fattyacids characteristic to Enterobacteriaceae, Bacteroidetes and Clostridia shows differences between the two digesters: their importance generally decreased under thermophilic conditions. The sulphate reducer marker (15:1 and 17:1) fatty acids are present in low quantity in both digesters.
机译：本工作的目的是比较中温和嗜热中试厌氧菌污泥消化池的微生物群落。为了研究社区，采用了独立的化学分类方法（RQ和PLFA分析）和T-RFLP。中温和嗜热中试消化池的微生物群落在存在的物种及其丰度方面都表现出相当大的差异。甲烷菌嗜热菌占主导地位，而甲烷菌属（Methanosaeta sp。）古细菌中的中温消化池。嗜热消化池中细菌的物种多样性降低。基于两个消化池中的醌模式，可以检测到硫酸盐还原呼吸细菌的优势。消化器群落的PLFA谱相似，尽管在次要成分中显示出特征差异。在嗜热消化器中，支链脂肪酸的含量略低，而革兰氏阳性菌较少。脂肪酸与肠杆菌科细菌，拟杆菌属和梭状芽胞杆菌的相对比例显示了两种消化器之间的差异：在高温条件下，它们的重要性通常会降低。硫酸盐还原剂标记物（15：1和17：1）的脂肪酸在两个消化池中的含量都很低。

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