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首页> 外文期刊>Acta Biochimica Polonica >The role of microtubules in electrotaxis of rat Walker carcinosarcoma WC256 cells
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The role of microtubules in electrotaxis of rat Walker carcinosarcoma WC256 cells

机译：微管在大鼠Walker癌肉瘤WC256细胞电静电中的作用

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The endogenous electric field may provide an important signal for directional cell migration during cancer metastasis but the mechanism of cell electrotaxis is poorly understood. It was postulated that microtubules play a central role in the polarization and directional migration of several types of cells. In this paper we investigated the role of microtubules in electrotaxis of rat Walker carcinosarcoma WC256 cells. We found that colchicine-stimulated disassembly of microtubules caused the formation of blebs instead of lamellipodia at the front of about 45% of cells. Most of the remaining cells contracted and became rounded or transformed into non-polar cells. Depolymerization of microtubules in both subpopulations of cells reduced the directionality of cell migration to about 50% of the control, but bleb-forming cells migrated much more efficiently than lamellipodia-forming cells. The analysis of microtubules architecture in the presence of an endogenous electric field showed that there is no relationship between the direction of migration and the polarization of microtubules. These results suggest that microtubules are not indispensable for electrotaxis of WC256 cells, however they may improve the directionality of cell migration.
机译：内源性电场可能为癌症转移过程中的定向细胞迁移提供重要信号，但对细胞电的机制了解甚少。据推测，微管在几种类型的细胞的极化和定向迁移中起着核心作用。在本文中，我们研究了微管在大鼠Walker癌肉瘤WC256细胞的静电中的作用。我们发现秋水仙碱刺激的微管拆卸引起大约45％的细胞前部形成小泡，而不是lamellipodia。其余大多数细胞收缩并变成圆形或转化为非极性细胞。细胞的两个亚群中的微管解聚将细胞迁移的方向性降低至对照的约50％，但形成气泡的细胞比形成lamellipodia的细胞更有效地迁移。在存在内源电场的情况下对微管结构的分析表明，迁移方向与微管的极化之间没有关系。这些结果表明，微管对于WC256细胞的电动并不是必不可少的，但是它们可以改善细胞迁移的方向性。

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