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首页> 外文期刊>Current Microbiology: An International Journal >Biocompatible PHB Production from Bacillus Species Under Submerged and Solid-State Fermentation and Extraction Through Different Downstream Processing
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Biocompatible PHB Production from Bacillus Species Under Submerged and Solid-State Fermentation and Extraction Through Different Downstream Processing

机译：在浸没和固态发酵和固态发酵和通过不同下游加工的提取的生物相容性PHB生产

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摘要

Catastrophic global accumulation of non-biodegradable plastic has led to efforts for production of alternative eco-friendly biopolymer. Here, we attempted to produce a biodegradable, cytocompatible and eco-friendly polyhydroxy-butyrate (PHB) from a pigmented Bacillus sp. C1 (2013) (KF626477) through submerged (SmF) and solid-state fermentation (SSF). Under SmF and SSF, 0.60 g l(-1) and 1.56 g l(-1) of PHB with 0.497 g l(-1) of yellow fluorescent pigment (YFP) was produced. Fourier transform infrared (FTIR) absorption bands at 1719-1720 cm(-1) indicate the presence of C=O group of PHB. Nuclear magnetic resonance (NMR) exhibited the typical chemical shift patterns of PHB, and crystallinity was confirmed from X-ray diffraction (XRD). The melting temperature (T-m), degradation temperature (T-d) and crystallinity (X-c) of extracted PHB were found to be 171 degrees C, 288 degrees C and 35%, respectively. FACS (Fluorescence-activated cell sorting) confirmed cytocompatibility of PHB at 400 mu g ml(-1) in mouse fibroblast line. Moreover, biodegradability and elevated cytocompatibility of the PHB produced through SSF make them highly potential biomaterials to be used as a drug delivery carrier in future.
机译：灾难性的全球非可生物降解塑料积累导致替代生态友好生物聚合物生产的努力。在此，我们试图从色素沉积的芽孢杆菌SP产生可生物降解的，细胞相容和环状的多羟基 - 丁酸酯（PHB）。 C1（2013）（KF626477）通过浸没（SMF）和固态发酵（SSF）。在SMF和SSF中，产生0.60g L（-1）和1.56g L（-1）的pHB，具有0.497g L（-1）的黄色荧光颜料（YFP）。傅里叶变换红外（FTIR）1719-1720cm（-1）的吸收带表示C = O基团的PHB。核磁共振（NMR）表现出pHB的典型化学变换模式，并从X射线衍射（XRD）证实结晶度。发现萃取PHB的熔化温度（T-M），降解温度（T-D）和结晶度（X-C）分别为171℃，288℃和35％。 FACS（荧光激活的细胞分选）证实了在小鼠成纤维细胞线中400μmGml（-1）的pHB的细胞相容性。此外，通过SSF产生的PHB的生物降解性和升高的细胞相容性使得它们在将来将其用作药物输送载体的高潜在的生物材料。

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