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首页> 外文期刊>Acta biomaterialia >An injectable scaffold: rhBMP-2-loaded poly(lactide-co-glycolide)/hydroxyapatite composite microspheres.
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An injectable scaffold: rhBMP-2-loaded poly(lactide-co-glycolide)/hydroxyapatite composite microspheres.

机译：可注射支架：RHBMP-2负载聚（丙交酯 - 共乙酰胺）/羟基磷灰石复合微球。

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摘要

Poly(lactide-co-glycolide)/hydroxyapatite(50/50) (PLGA/HA(50/50)) composite microspheres were fabricated and treated with a mixture of 0.25M NaOH aqueous solution and ethanol (v/v=1/1) at 37 degrees C. The properties of untreated and treated PLGA/HA(50/50) composite microspheres were determined and compared. The results showed that the surface roughness, HA content and hydrophilicity of the treated PLGA/HA(50/50) composite microspheres increased with treatment time. However, the treatment time should be kept within 2h in order to maintain the shape of the PLGA/HA(50/50) microspheres. At the same time, a degradation study showed that both the untreated and treated microspheres degraded gradually with time, with the treated microspheres degrading faster in the first 4 weeks. The rhBMP-2-loaded PLGA/HA(50/50) composite microspheres were prepared by solution dipping treated PLGA/HA(50/50) composite microspheres. Mouse OCT-1 osteoblast-like cells were cultured on the untreated, treated and rhBMP-2-loaded PLGA/HA(50/50) composite microspheres and the cell affinity of the various microspheres was assessed and compared. It was found that the surface-treated PLGA/HA(50/50) composite microspheres clearly promoted osteoblast attachment, proliferation and alkaline phosphatase activity. It was considered that the hydrophilicity, osteoconductivity and surface roughness were increased by the increase in the HA component, which facilitated cell growth. Moreover, the rhBMP-2 loaded on the treated PLGA/HA(50/50) composite microspheres could be slowly released and further enhanced osteoblast differentiation. The good cell affinity and enhanced osteogenic potential of the rhBMP-2-loaded PLGA/HA composite microspheres indicate that they could be used as an injectable scaffold.
机译：聚（丙交酯 - 共乙酰胺）/羟基磷灰石（50/50）（PLGA / HA（50/50））复合微球，并用0.25m NaOH水溶液和乙醇的混合物处理（v / v = 1/1 ）在37摄氏度下测定未处理和处理的PLGA / HA（50/50）复合微球的性质。结果表明，经处理的PLGA / HA（50/50）复合微球的表面粗糙度，HA含量和亲水性随治疗时间而增加。然而，应在2h内保持处理时间，以保持PLGA / HA（50/50）微球的形状。同时，降解研究表明，未处理和处理的微球两者随时间逐渐降解，处理过的微球在前4周内降低更快。通过溶液浸渍处理的PLGA / HA（50/50）复合微球制备RHBMP-2负载PLGA / HA（50/50）复合微球。将小鼠OCT-1对骨细胞样细胞培养在未处理的，处理的和RHBMP-2负载的PLGA / HA（50/50）复合微球中，并评估各种微球的细胞亲和力并进行比较。发现表面处理的PLGA / HA（50/50）复合微球明显促进了成骨细胞附着，增殖和碱性磷酸酶活性。认为亲水性，骨导电性和表面粗糙度增加了HA组分的增加，促进了细胞生长。此外，可以缓慢释放在处理过的PLGA / HA（50/50）复合微球上的RHBMP-2，进一步增强了成骨细胞分化。 RHBMP-2负载PLGA / HA复合微球的良好细胞亲和力和增强的成骨电位表明它们可以用作可注射支架。

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  • 来源
    《Acta biomaterialia》 |2010年第2期|共11页
  • 作者

    Shen H; Hu X; Yang;

  • 作者单位

    Institute of Chemistry Chinese Academy of Sciences Center for Molecular Sciences Beijing China.;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物医学工程;
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