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首页> 外文期刊>Acta biomaterialia >Osteointegration of titanium implant is sensitive to specific nanostructure morphology
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Osteointegration of titanium implant is sensitive to specific nanostructure morphology

机译：钛植入的骨核对敏感性对特异性纳米结构形态敏感

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An important aspect of orthopedic implant integration is the enhancement of functional activity of osteoblasts at the tissue-implant interface without any fibrous tissue intervention. Nanostructured implant surfaces are known to enhance osteoblast activity. Previously, we have reported a simple hydrothermal method for the fabrication of non-periodic nanostructures (nanoscaffold, nanoleaves and nanoneedles) on titanium implants showing good biocompatibility and a distinct osteoblast response in vitro in terms of osteoblast adhesion to the surface. In the present work, these nanostructures have been evaluated for their detailed in vitro cellular response as well as in vivo osteointegration. Our studies showed that a specific surface nanomorphology, viz. nanoleaves, which is a network of vertically aligned, non-periodic, leaf-like structures with thickness in the nanoscale, provided a distinct increase in osteoblast cell proliferation, alkaline phosphatase (ALP) activity and collagen synthesis compared to several other types of nanomorphology, such as nanotubes, nanoscaffold and nanoneedles (rods). Gene expression analysis of ALP, osteocalcin, collagen, decorin and Runx2 showed ～20- to 40-fold up-regulation on the leaf-like topography. Cytoskeletal arrangement studies on this substrate again revealed a unique response with favorable intracellular protein expressions of vinculin, FAK and src. In vivo osteointegration study over 12 weeks on rat model (Sprague-Dawley) showed early-stage bone formation (60% bone contact by week 2 and ～85% by week 8, p < 0.01) in the leaf-like nanopattern, without any inflammatory cytokine production.
机译：整形外科植入物整合的一个重要方面是增强组织植入界面处的成骨细胞的功能活性，而没有任何纤维组织介入。已知纳米结构植入物表面增强成骨细胞活性。以前，我们已经报道了一种简单的水热方法，用于在钛植入物上制造非周期性纳米结构（Nanoscold，纳米物体和纳米块），其在表面上呈现出良好的生物相容性和体外的不同的成骨细胞反应。在本作的工作中，已经评估了这些纳米结构，用于它们详细的体外细胞反应以及体内骨质骨折。我们的研究表明，特定的表面纳米形态，VIZ。作为纳米尺度厚度的垂直对准，非周期性，叶状结构的纳米杆，提供了与几种其他类型的纳米形态相比的成骨细胞增殖，碱性磷酸酶（ALP）活性和胶原合成的不同增加，如纳米管，纳米支型和纳米杆（杆）。 ALP，骨钙素，胶原蛋白，装饰汀和RUNX2的基因表达分析显示叶状地形上的〜20至40倍上调。对该基质的细胞骨架布置研究再次揭示了vinculin，Fak和Src的有利细胞内蛋白表达的独特反应。在大鼠模型（Sprague-Dawley）上超过12周的体内骨整向性研究（Sprague-Dawley）显示出早期的骨形成（骨接触60％骨接触，在叶状纳米透舱图中，没有任何情况下的第8周，P <0.01），没有任何炎症细胞因子生产。

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