• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta biomaterialia >Osteointegration of titanium implant is sensitive to specific nanostructure morphology
【24h】

Osteointegration of titanium implant is sensitive to specific nanostructure morphology

机译：钛植入物的骨整合对特定的纳米结构形态敏感

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An important aspect of orthopedic implant integration is the enhancement of functional activity of osteoblasts at the tissue-implant interface without any fibrous tissue intervention. Nanostructured implant surfaces are known to enhance osteoblast activity. Previously, we have reported a simple hydrothermal method for the fabrication of non-periodic nanostructures (nanoscaffold, nanoleaves and nanoneedles) on titanium implants showing good biocompatibility and a distinct osteoblast response in vitro in terms of osteoblast adhesion to the surface. In the present work, these nanostructures have been evaluated for their detailed in vitro cellular response as well as in vivo osteointegration. Our studies showed that a specific surface nanomorphology, viz. nanoleaves, which is a network of vertically aligned, non-periodic, leaf-like structures with thickness in the nanoscale, provided a distinct increase in osteoblast cell proliferation, alkaline phosphatase (ALP) activity and collagen synthesis compared to several other types of nanomorphology, such as nanotubes, nanoscaffold and nanoneedles (rods). Gene expression analysis of ALP, osteocalcin, collagen, decorin and Runx2 showed ～20- to 40-fold up-regulation on the leaf-like topography. Cytoskeletal arrangement studies on this substrate again revealed a unique response with favorable intracellular protein expressions of vinculin, FAK and src. In vivo osteointegration study over 12 weeks on rat model (Sprague-Dawley) showed early-stage bone formation (60% bone contact by week 2 and ～85% by week 8, p < 0.01) in the leaf-like nanopattern, without any inflammatory cytokine production.
机译：整形外科植入物整合的一个重要方面是无需任何纤维组织干预，即可增强成骨细胞在组织-植入物界面处的功能活性。已知纳米结构植入物表面可增强成骨细胞活性。以前，我们已经报道了一种简单的水热法，用于在钛植入物上制造非周期性纳米结构（纳米脚手架，纳米叶和纳米针），在成骨细胞对表面的粘附方面表现出良好的生物相容性和独特的成骨反应。在目前的工作中，已经对这些纳米结构的详细的体外细胞反应以及体内的骨整合进行了评估。我们的研究表明，特定的表面纳米形态，即。纳米叶是垂直排列的非周期性叶状结构的网络，厚度为纳米级，与其他几种类型的纳米形态相比，成骨细胞增殖，碱性磷酸酶（ALP）活性和胶原蛋白合成显着增加，例如纳米管，纳米支架和纳米针（棒）。 ALP，骨钙素，胶原蛋白，decorin和Runx2的基因表达分析显示，在叶状形貌上表达上调约20至40倍。在该底物上的细胞骨架排列研究再次揭示了独特的应答，其中长链蛋白，FAK和src具有良好的细胞内蛋白表达。在大鼠模型（Sprague-Dawley）上进行的为期12周的体内骨整合研究显示，叶状纳米模式的早期骨形成（第2周的骨接触率为60％，第8周的骨接触率为〜85％，p <0.01），没有任何形成炎性细胞因子的产生。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号