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首页> 外文期刊>Acta biomaterialia >Autologous vs. allogenic mesenchymal progenitor cells for the reconstruction of critical sized segmental tibial bone defects in aged sheep.
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Autologous vs. allogenic mesenchymal progenitor cells for the reconstruction of critical sized segmental tibial bone defects in aged sheep.

机译：自体与同种异体间充质祖细胞重建脑绵的临界大小胫骨骨缺损。

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摘要

Mesenchymal progenitor cells (MPCs) represent an attractive cell population for bone tissue engineering. Their special immunological characteristics suggest that MPCs may be used in allogenic applications. The objective of this study was to compare the regenerative potential of autologous vs. allogenic MPCs in an ovine critical size segmental defect model. Ovine MPCs were isolated from bone marrow aspirates, expanded and cultured with osteogenic medium for 2weeks before implantation. Autologous and allogenic transplantation was performed using the cell-seeded scaffolds and unloaded scaffolds, while the application of autologous bone grafts served as a control group (n=6). Bone healing was assessed 12weeks after surgery by radiology, microcomputed tomography, biomechanical testing and histology. Radiology, biomechanical testing and histology revealed no significant differences in bone formation between the autologous and allogenic groups. Both cell groups showed more bone formation than the scaffold alone, whereas the biomechanical data showed no significant differences between the cell groups and the unloaded scaffolds. The results of the study suggest that scaffold-based bone tissue engineering using allogenic cells offers the potential for an off-the-shelf product. Thus the results of this study serve as an important baseline for translation of the assessed concepts into clinical applications.
机译：间充质祖细胞（MPC）代表骨组织工程的有吸引力的细胞群。它们的特殊免疫特性表明MPC可用于同种异体应用。本研究的目的是比较绵延临界大小分段缺陷模型中自体与同种异体MPCs的再生潜力。从骨髓抽吸物中分离绵羊MPC，在植入前用骨髓培养基进行膨胀和培养。使用细胞播种支架和卸载支架进行自体和同种异体移植，同时施用自体骨移植物作为对照组（n = 6）。通过放射学，微锁定断层扫描，生物力学检测和组织学，在手术后进行12周评估骨愈合。放射学，生物力学测试和组织学揭示了自体和同种异体基团之间的骨形成的显着差异。两个细胞基团的单独显示比支架更多的骨形成，而生物力学数据显示在细胞基团和卸载的支架之间没有显着差异。该研究的结果表明，使用同种异体电池的基于支架的骨组织工程提供了架子产品的潜力。因此，该研究的结果作为评估概念转化为临床应用的重要基线。

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