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首页> 外文期刊>Acta biomaterialia >Control of proliferation and osteogenic differentiation of human dental-pulp-derived stem cells by distinct surface structures.
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Control of proliferation and osteogenic differentiation of human dental-pulp-derived stem cells by distinct surface structures.

机译：不同表面结构控制人牙牙髓衍生干细胞的增殖和骨质分化。

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The ability to control the behavior of stem cells provides crucial benefits, for example, in tissue engineering and toxicity/drug screening, which utilize the stem cell's capacity to engineer new tissues for regenerative purposes and the testing of new drugs in vitro. Recently, surface topography has been shown to influence stem cell differentiation; however, general trends are often difficult to establish due to differences in length scales, surface chemistries and detailed surface topographies. Here we apply a highly versatile screening approach to analyze the interplay of surface topographical parameters on cell attachment, morphology, proliferation and osteogenic differentiation of human mesenchymal dental-pulp-derived stem cells (DPSCs) cultured with and without osteogenic differentiation factors in the medium (ODM). Increasing the inter-pillar gap size from 1 to 6 μm for surfaces with small pillar sizes of 1 and 2 μm resulted in decreased proliferation and in more elongated cells with long pseudopodial protrusions. The same alterations of pillar topography, up to an inter-pillar gap size of 4 μm, also resulted in enhanced mineralization of DPSCs cultured without ODM, while no significant trend was observed for DPSCs cultured with ODM. Generally, cells cultured without ODM had a larger deposition of osteogenic markers on structured surfaces relative to the unstructured surfaces than what was found when culturing with ODM. We conclude that the topographical design of biomaterials can be optimized for the regulation of DPSC differentiation and speculate that the inclusion of ODM alters the ability of the cells to sense surface topographical cues. These results are essential in order to transfer the use of this highly proliferative, easily accessible stem cell into the clinic for use in cell therapy and regenerative medicine.
机译：控制干细胞行为的能力为例如组织工程和毒性/药物筛选提供了至关重要的益处，该益处利用干细胞的能力来制造用于再生目的的新组织和体外新药的测试。最近，表面形貌已显示影响干细胞分化;然而，由于长度尺度，表面化学品和详细的表面形貌的差异，通常难以建立。在这里，我们应用一种高度通用的筛选方法来分析用培养基中培养的人间充质牙髓衍生的干细胞（DPSC）的细胞附着，形态，增殖和骨质发生分化的表面形貌参数的相互作用。（ ODM）。对于小柱尺寸为1和2μm的表面增加柱间隙大小从1至6μm导致引起的增殖降低，并且具有长假透过突起的细长细胞。柱形的相同改变，直到柱间隙尺寸为4μm，也导致了没有ODM培养的DPSCs的增强矿化，而用ODM培养的DPSC没有显着趋势。通常，在没有ODM的情况下培养的细胞在相对于非结构化表面上的结构化表面上的骨质原标记物的较大沉积而不是用ODM培养时发现的。我们得出结论，生物材料的地形设计可针对DPSC分化的调节进行优化，并推测包含ODM的能力使细胞感测表面地形提示的能力。这些结果对于将这种高增殖性，易于无障碍的干细胞转移到用于细胞疗法和再生医学的临床中的使用是必不可少的。

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