• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta biomaterialia >Control of proliferation and osteogenic differentiation of human dental-pulp-derived stem cells by distinct surface structures.
【24h】

Control of proliferation and osteogenic differentiation of human dental-pulp-derived stem cells by distinct surface structures.

机译：通过独特的表面结构控制人牙髓衍生干细胞的增殖和成骨分化。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The ability to control the behavior of stem cells provides crucial benefits, for example, in tissue engineering and toxicity/drug screening, which utilize the stem cell's capacity to engineer new tissues for regenerative purposes and the testing of new drugs in vitro. Recently, surface topography has been shown to influence stem cell differentiation; however, general trends are often difficult to establish due to differences in length scales, surface chemistries and detailed surface topographies. Here we apply a highly versatile screening approach to analyze the interplay of surface topographical parameters on cell attachment, morphology, proliferation and osteogenic differentiation of human mesenchymal dental-pulp-derived stem cells (DPSCs) cultured with and without osteogenic differentiation factors in the medium (ODM). Increasing the inter-pillar gap size from 1 to 6 μm for surfaces with small pillar sizes of 1 and 2 μm resulted in decreased proliferation and in more elongated cells with long pseudopodial protrusions. The same alterations of pillar topography, up to an inter-pillar gap size of 4 μm, also resulted in enhanced mineralization of DPSCs cultured without ODM, while no significant trend was observed for DPSCs cultured with ODM. Generally, cells cultured without ODM had a larger deposition of osteogenic markers on structured surfaces relative to the unstructured surfaces than what was found when culturing with ODM. We conclude that the topographical design of biomaterials can be optimized for the regulation of DPSC differentiation and speculate that the inclusion of ODM alters the ability of the cells to sense surface topographical cues. These results are essential in order to transfer the use of this highly proliferative, easily accessible stem cell into the clinic for use in cell therapy and regenerative medicine.
机译：控制干细胞行为的能力提供了至关重要的好处，例如，在组织工程和毒性/药物筛选中，利用了干细胞为再生目的改造新组织的能力以及体外新药的测试。最近，表面形貌已被证明会影响干细胞的分化。然而，由于长度比例，表面化学和详细的表面形貌的差异，通常难以确定总体趋势。在这里，我们采用了一种高度通用的筛选方法来分析表面地形参数对人间充质牙髓衍生干细胞（DPSC）的细胞附着，形态，增殖和成骨分化的相互作用，在培养基中培养和不培养成骨分化因子（ ODM）。对于具有1和2μm小柱尺寸的表面，将柱间间隙尺寸从1μm增加到6μm会导致增殖减少，并且具有较长的伪足突起的细胞会更长。相同的立柱形貌变化（最大柱间间隙大小为4μm）也导致不使用ODM培养的DPSC的矿化作用增强，而使用ODM培养的DPSC则没有观察到明显的趋势。通常，与使用ODM培养相比，在没有ODM的情况下培养的细胞相对于非结构化表面，其成骨标记物在结构化表面的沉积更大。我们得出的结论是，可以优化生物材料的地形设计以调节DPSC分化，并推测ODM的加入会改变细胞感知表面地形线索的能力。这些结果对于将这种高度增殖，易于获取的干细胞的用途转移到临床上用于细胞疗法和再生医学至关重要。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号