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首页> 外文期刊>Acta Biochimica Polonica >Biodiversity of Lactococcus lactis bacteriophages in Polish dairy environment
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Biodiversity of Lactococcus lactis bacteriophages in Polish dairy environment

机译：波兰乳业环境中乳酸乳球菌噬菌体的生物多样性

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摘要

We present here the results of an exploration of the bacteriophage content of dairy wheys collected from milk plants localized in various regions of Poland. Thirty-three whey samples from 17 regions were analyzed and found to contain phages active against L. lactis strains. High phage titer in all whey samples suggested phage-induced lysis to be the main cause of fermentation failures. In total, over 220 isolated phages were examined for their restriction patterns, genome sizes, genetic groups of DNA homology, and host ranges. Based on DNA digestions the identified phages were classified into 34 distinct DNA restriction groups. Phage genome sizes were estimated at 14-35 kb. Multiplex PCR analysis established that the studied phages belong to two out of the three main lactococcal phage types - c2 and 936, while P335-type phages were not detected. Yet, analyses of bacterial starter strains revealed that the majority of them are lysogenic and carry prophages of P335-type in their chromosome. Phage geographical distribution and host range are additionally discussed.
机译：我们在这里介绍了从位于波兰各个地区的牛奶厂收集的乳清乳清的噬菌体含量的研究结果。分析了来自17个地区的33个乳清样品，发现它们含有对乳酸乳球菌菌株具有活性的噬菌体。所有乳清样品中的高噬菌体滴度表明，噬菌体诱导的裂解是发酵失败的主要原因。总共检查了220多个分离的噬菌体的限制模式，基因组大小，DNA同源性的遗传组和宿主范围。基于DNA消化，鉴定出的噬菌体被分为34个不同的DNA限制组。噬菌体基因组大小估计为14-35 kb。多重PCR分析确定，所研究的噬菌体属于三种主要的乳球菌噬菌体类型-c2和936中的两种，而未检测到P335型噬菌体。然而，对细菌发酵菌株的分析表明，它们大多数是溶原性的，在其染色体上带有P335型噬菌体。还讨论了噬菌体的地理分布和寄主范围。

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