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首页> 外文期刊>Crystal growth & design >Obtainment of Spherical-Shaped Calcite Crystals Induced by Intramineral Proteins Isolated from Eggshells of Ostrich and Emu
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Obtainment of Spherical-Shaped Calcite Crystals Induced by Intramineral Proteins Isolated from Eggshells of Ostrich and Emu

机译:从鸵鸟和E的蛋壳中分离的矿物质蛋白诱导球形方解石晶体的获得

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摘要

In this work, we have performed a set of experiments to study how intramineral proteins affected the growth of calcite crystals and induced spherical growth. Intramineral proteins were first isolated from ostrich and emu eggshells and then purified by liquid chromatography. Samples of these proteins were analyzed by means of cyclic voltammetry to check their selectivity for carbonate ions. Then they underwent assays in solution through dynamic light scattering (DLS) to help us establish their homogeneity and stability (whether individually or in pairs) in the presence of carbonate ions. This was followed by in vitro crystallization experiments to determine how they affected the morphology of calcite crystals interacting with the intramineral proteins. Finally, scanning electron microscopy (SEM) showed that the morphology of the calcite crystals was strongly modified in the presence of these intramineral proteins.
机译:在这项工作中,我们进行了一组实验,以研究矿物质蛋白如何影响方解石晶体的生长并诱导球形生长。首先从鸵鸟和e壳中分离出矿物质蛋白,然后通过液相色谱法纯化。通过循环伏安法分析这些蛋白质的样品,以检查其对碳酸根离子的选择性。然后,他们通过动态光散射(DLS)在溶液中进行测定,以帮助我们在存在碳酸根离子的情况下确定其均一性和稳定性(无论是单独还是成对)。随后进行体外结晶实验,以确定它们如何影响与矿物蛋白相互作用的方解石晶体的形态。最后,扫描电子显微镜(SEM)显示,在这些矿物质蛋白的存在下,方解石晶体的形态得到了强烈修饰。

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