A salt stress induced up-regulation of chloroplast cDNA sequence from canola shows similarity to carcinoma related-DNA sequences

Saadia M.; Jamil A.; Ashraf M.; Iqbal T.; Aicraw N. A.; Mushtaq Z.; Shukat I.

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A salt stress induced up-regulation of chloroplast cDNA sequence from canola shows similarity to carcinoma related-DNA sequences

机译：盐应激诱导从油菜的叶绿体cDNA序列的上调表现出与癌相关DNA序列的相似性

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In the present study, differential expression of key DNA sequences responsive to salt stress in plant seedlings subjected to 150 mm NaCl, was investigated in a salt-sensitive canola line (Cyclone) by differential display reverse transcription PCR (DDRT-PCR). A canola cDNA fragment (SCA(1)H(27)) was isolated using an arbitrary primer that was up-regulated under stress, but showed no similarity to any of known plant genes from the nucleotide database through BLAST analysis. However, it showed significant similarity to the chloroplast DNA sequences thereby confirming its similarity. The predicted amino acid sequence of the isolated cDNA sequence showed significant similarity to the carcinoma-related and the catalase-peroxidase protein sequences of species other than Brassicaceae using the SwissPort and PDB protein database. The results showed the supposition of induction of carcinoma-related DNA sequences in the chloroplast of plant cells under salt stress. Further proteomic studies would be helpful in identifying definite function of this novel canola cDNA upregulated in relation to salt sensitivity.

机译：在本研究中，通过差分显示逆转录PCR（DDRT-PCR）在盐敏性DAMLA线（Cyclone）中响应于经过150mM NaCl的植物幼苗中响应于盐胁迫的关键DNA序列的差异表达。使用在应力下上调的任意引物分离了CanoLA cDNA片段（SCA（1）H（27）），但通过BLAST分析显示与核苷酸数据库中的任何已知植物基因的相似性。然而，它表现出与叶绿体DNA序列的显着相似度，从而确认其相似性。预测的分离CDNA序列的氨基酸序列与使用Swissport和PDB蛋白质数据库的甘草植物以外的物种与癌酶相关和过氧化氢酶过氧化物酶蛋白序列显着相似。结果表明，盐胁迫下植物细胞叶绿体中癌癌相关DNA序列的诱导。进一步的蛋白质组学研究将有助于鉴定该新型CANOLA cDNA的定义功能，同等于盐敏感性上调。

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《Agrochimica》 |2017年第3期|共15页
作者
Saadia M.; Jamil A.; Ashraf M.; Iqbal T.; Aicraw N. A.; Mushtaq Z.; Shukat I.;
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作者单位

Univ Sargodha Dept Chem Sargodha Pakistan;

Univ Agr Faisalabad Dept Chem &

Biochem Faisalabad Pakistan;

Pakistan Sci Fdn Islamabad Pakistan;

Univ Agr Faisalabad Dept Chem &

Biochem Faisalabad Pakistan;

GC Univ Faisalabad Faisalabad Pakistan;

Univ Agr Faisalabad Dept Chem &

Biochem Faisalabad Pakistan;

Univ Agr Faisalabad Dept Chem &

Biochem Faisalabad Pakistan;

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原文格式 PDF
正文语种 eng
中图分类农业化学;
关键词
canola; chloroplast cDNA; salt stress; biotechnology;

机译：油菜;叶绿体cDNA;盐胁迫;生物技术;

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专利

1. A salt stress induced up-regulation of chloroplast cDNA sequence from canola shows similarity to carcinoma related-DNA sequences [J] . Saadia M., Jamil A., Ashraf M., Agrochimica . 2017,第3期

机译：盐应激诱导从油菜的叶绿体cDNA序列的上调表现出与癌相关DNA序列的相似性
2. Prediction whether a human cDNA sequence contains initiation codon by combining statistical information and similarity with protein sequences. [J] . Nishikawa T, Ota T, Isogai T Bioinformatics . 2000,第11期

机译：通过将统计信息和与蛋白质序列的相似性相结合，预测人类cDNA序列是否包含起始密码子。
3. cDNA representational difference analysis of differentially expressed cDNA sequences in human nasopharyngeal carcinoma [J] . Zhan Fenghuang, Cao Li, Bin Lianghua Chinese Medical Journal . 1999,第6期

机译：人鼻咽癌中差异表达cDNA序列的cDNA代表性差异分析
4. A Chaining Algorithm for Mapping cDNA Sequences to Multiple Genomic Sequences [C] . Mohamed Abouelhoda International Conference on String Processing and Information Retrieval(SPIRE 2007); 20071029-31; Santiago(CL) . 2007

机译：一种将cDNA序列映射到多个基因组序列的链接算法
5. Sequence analysis of Ustilago maydis cDNA libraries: Genome annotation and nutrient stress response. [D] . Ho, Eric Chun Hei. 2006

机译：玉米Us乌贼属cDNA文库的序列分析：基因组注释和营养胁迫响应。
6. Expression during Salt Stress and Nucleotide Sequence of cDNA for Ferredoxin-NADP+ Reductase from Mesembryanthemum crystallinum [O] . Christine B. Michalowski, Jürgen M. Schmitt, Hans J. Bohnert 1989

机译：盐菊中铁氧还蛋白-NADP +还原酶的盐胁迫过程中的表达及cDNA的核苷酸序列
7. Osgstu3 and osgtu4, encoding tau class glutathione S-transferases, are heavy metal- and hypoxic stress-induced and differentially salt stress-responsive in rice roots11The nucleotide sequences reported in this paper have been submitted to the EMBL, GenBank and DDBJ nucleotide sequence databases under the accession numbers AY271619 and AY271620. [O] . Moons Ann 2003

机译：编码tau类谷胱甘肽S-转移酶的Osgstu3和osgtu4是重金属和低氧胁迫诱导的，并且在盐根中具有不同的盐胁迫响应11。本文报道的核苷酸序列已提交给EMBL，GenBank和DDBJ核苷酸序列数据库，登录号AY271619和AY271620。
8. Isolation and Sequencing of a cDNA Clone Encoding Lysosomal Membrane Glycoprotein mLAMP-1: Sequence Similarity to Proteins Bearing Onco-Differentiation Antigens [R] . Chenn, J. W., Cha, Y., Yukselt, K. U., 1988

机译：溶酶体膜糖蛋白mLamp-1的cDNa克隆的分离和测序：与携带分化抗原的蛋白质的序列相似性

A salt stress induced up-regulation of chloroplast cDNA sequence from canola shows similarity to carcinoma related-DNA sequences

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