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首页> 外文期刊>AIDS Research and Human Retroviruses >ARCHITECT HIV Combo Ag/Ab and RealTime HIV-1 Assays Detect Diverse HIV Strains in Clinical Specimens
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ARCHITECT HIV Combo Ag/Ab and RealTime HIV-1 Assays Detect Diverse HIV Strains in Clinical Specimens

机译：建筑师HIV组合AG / AB和实时HIV-1测定检测临床标本中不同的HIV菌株

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摘要

Periodic evaluation of the impact of viral diversity on diagnostic tests is critical to ensure current technologies are keeping pace with viral evolution. To determine whether HIV diversity impacts the ARCHITECT HIV Combo Ag/Ab (HIV Combo) or RealTime HIV-1 (RT) assays, a set of N = 199 HIV clinical specimens from Cameroon, Senegal, Saudi Arabia, and Thailand were sequenced and tested in both assays. The panel included historical groups N and P specimens and a newly identified group N specimen. These and specimens classified as H, U (unclassified)/URF (unique recombinant form), CRF (circulating recombinant form) 01, 02, 06, 09, 11, 13, 18, 22, 37, and 43 were detected by both the RT assay (1.75–6.84 log copies/ml) and the HIV Combo assay (3.26–1121.96 sample to cutoff ratios). Sequence alignment identified 3 or fewer mismatches to the RT assay oligos in 82.4% of samples. Altogether, these data demonstrate the HIV Combo and RT assays detect diverse strains of HIV in clinical specimens.
机译：定期评估病毒多样性对诊断测试的影响对于确保当前技术与病毒演化保持步伐是至关重要的。为了确定艾滋病毒多样性是否会影响建筑师HIV组合AG / AB（HIV组合）或实时HIV-1（RT）测定，从喀麦隆，塞内加尔，沙特阿拉伯和泰国进行了一组N = 199艾滋病毒临床标本并进行了测序和测试在两个测定中。该面板包括历史组和P样本和新鉴定的N标本。分类为H，U（未分类）/ URF（独特的重组形式），CrF（循环重组形式）01,02,06,09,11,13,18,22,37和43的标本被两者检测到RT测定（1.75-6.84对数拷贝/ mL）和HIV组合测定（3.26-1121.96样品到截止比率）。序列对准在82.4％样品中鉴定到RT测定寡核苷酸的3或更少的不匹配。总共，这些数据证明了HIV组合和RT测定检测临床标本中的不同育虫菌株。

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    Rodgers Mary A.; Vallari Ana S.; Yamaguchi Julie; Holzmayer Vera; Harris Barbara; Toure-Kane Coumba; Mboup Souleymane; Badreddine Samar; McArthur Carole; Ndembi Nicaise; Mbanya Dora; Kaptue Lazare; Cloherty Gavin;

  • 作者单位

    Infectious Disease Research Abbott Diagnostics Abbott Park Illinois.;

    Infectious Disease Research Abbott Diagnostics Abbott Park Illinois.;

    Infectious Disease Research Abbott Diagnostics Abbott Park Illinois.;

    Infectious Disease Research Abbott Diagnostics Abbott Park Illinois.;

    Infectious Disease Research Abbott Diagnostics Abbott Park Illinois.;

    Institut de Recherche en Santé de Surveillance Epidemiologique et de Formations Dakar Senegal.;

    Institut de Recherche en Santé de Surveillance Epidemiologique et de Formations Dakar Senegal.;

    Infection Control Unit King Faisal Specialist Hospital and Research Center Jeddah Saudi Arabia.;

    Department of Oral and Craniofacial Sciences School of Dentistry University of Missouri-Kansas;

    Laboratory Research Department Institute of Human Virology Abuja Nigeria.;

    Department of Hematology Université de Yaoundé I Yaoundé Cameroon.;

    Laboratoire D'Hematologie Université des Montagnes Bangangté Cameroon.;

    Infectious Disease Research Abbott Diagnostics Abbott Park Illinois.;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 传染病;
  • 关键词

    HIV diversity; diagnostics; serology; nucleic acid test;

    机译：艾滋病毒多样性;诊断;血清学;核酸试验;

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