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首页> 外文期刊>Alcohol >Simultaneous wireless and high-resolution detection of nucleus accumbens shell ethanol concentrations and free motion of rats upon voluntary ethanol intake
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Simultaneous wireless and high-resolution detection of nucleus accumbens shell ethanol concentrations and free motion of rats upon voluntary ethanol intake

机译：同时无线和高分辨率检测核心腺壳乙醇浓度和大鼠自主乙醇摄入量的自由运动

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摘要

Highly sensitive detection of ethanol concentrations in discrete brain regions of rats voluntarily accessing ethanol, with high temporal resolution, would represent a source of greatly desirable data in studies devoted to understanding the kinetics of the neurobiological basis of ethanol's ability to impact behavior. In the present study, we present a series of experiments aiming to validate and apply an original hightech implantable device, consisting of the coupling, for the first time, of an amperometric biosensor for brain ethanol detection, with a sensor for detecting the microvibrations of the animal. This device allows the real-time comparison between the ethanol intake, its cerebral concentrations, and their effect on the motion when the animal is in the condition of voluntary drinking. To this end, we assessed in vitro the efficiency of three different biosensor designs loading diverse alcohol oxidase enzymes (AOx) obtained from three different AOx-donor strains: Hansenula polymorpha, Candida boidinii, and Pichia pastoris. In vitro data disclosed that the devices loading H. polymorpha and C. boidinii were similarly efficient (respectively, linear region slope [IRS]: 1.98 +/- 0.07 and 1.38 +/- 0.04 nA/mM) but significantly less than the P. pastoris-loaded one (LRS: 7.57 +/- 0.12 nA/mM). The in vivo results indicate that this last biosensor design detected the rise of ethanol in the nucleus accumbens shell (AcbSh) after 15 minutes of voluntary 10% ethanol solution intake. At the same time, the microvibration sensor detected a significant increase in the rat's motion signal. Notably, both the biosensor and microvibration sensor described similar and parallel time-dependent U-shaped curves, thus providing a highly sensitive and time-locked high-resolution detection of the neurochemical and behavioral kinetics upon voluntary ethanol intake. The results overall indicate that such a dual telemetry unit represents a powerful device which, implanted in different brain areas, may boost further investigations on the neurobiological mechanisms that underlie ethanol induced motor activity and reward. (C) 2019 Elsevier Inc. All rights reserved.
机译：高敏感性检测在自愿进入乙醇的大鼠离散脑区中的乙醇浓度，具有高季度分辨率，可以代表致力于理解乙醇的神经生理学基础的动力学的研究中有着极大理想的数据来源。在本研究中，我们提出了一系列旨在验证和应用原始Hightech可植入装置的实验，该装置首次由脑乙醇检测的脑乙醇检测组成，具有用于检测微臂的传感器动物。该装置允许在动物处于自愿饮用条件时，乙醇摄入量，脑浓度与其对运动的影响进行实时比较。为此，我们在体外评估了三种不同的生物传感器设计的效率加载了由三种不同的Axox供体菌株获得的多种酒精氧化酶（AOX）：Hansenula多晶晶晶素，念珠菌Boidinii和Pichia Pastoris。在体外数据公开的是，装载H.多晶型和C.Boidinii的装置是类似的有效性的（分别的线性区域坡度[IRS]：1.98 +/- 0.07和1.38 +/- 0.04 Na / mm），但明显小于P. Pastoris Loaded一（LRS：7.57 +/- 0.12 / mm）。体内结果表明，在自愿10％乙醇溶液摄入量的15分钟后，最后一次生物传感器设计检测到核骨壳（ACBSH）中乙醇的升高。同时，微纤维传感器检测到大鼠运动信号的显着增加。值得注意的是，生物传感器和微纤维传感器都描述了相似且平行的时间依赖性U形曲线，从而在自愿乙醇摄入时提供了神经化学和行为动力学的高度敏感和时滞的高分辨率检测。结果总体上表明，这种双遥测单元代表了一种植入在不同脑区域的强大装置，可以提高对乙醇诱导的电动机活动和奖励的神经生物学机制进一步研究。 （c）2019 Elsevier Inc.保留所有权利。

著录项

  • 来源
    《Alcohol》 |2019年第2019期|共10页
  • 作者

    Rocchitta G.; Peana A. T.; Bazzu G.; Cossu A.; Carta S.; Arrigo P.; Bacciu A.; Migheli R.; Farina D.; Zinellu M.; Acquas E.; Serra P. A.;

  • 作者单位

    Univ Sassari Sect Pharmacol Dept Med Surg &

    Expt Sci Vle S Pietro 43-B I-07100 Sassari Italy;

    Univ Sassari Dept Chem &

    Pharm Via Muroni 23 I-07100 Sassari Italy;

    Univ Sassari Sect Pharmacol Dept Med Surg &

    Expt Sci Vle S Pietro 43-B I-07100 Sassari Italy;

    Univ Sassari Sect Pharmacol Dept Med Surg &

    Expt Sci Vle S Pietro 43-B I-07100 Sassari Italy;

    Univ Sassari Sect Pharmacol Dept Med Surg &

    Expt Sci Vle S Pietro 43-B I-07100 Sassari Italy;

    Univ Sassari Sect Pharmacol Dept Med Surg &

    Expt Sci Vle S Pietro 43-B I-07100 Sassari Italy;

    Univ Sassari Sect Pharmacol Dept Med Surg &

    Expt Sci Vle S Pietro 43-B I-07100 Sassari Italy;

    Univ Sassari Sect Pharmacol Dept Med Surg &

    Expt Sci Vle S Pietro 43-B I-07100 Sassari Italy;

    Univ Sassari Sect Pharmacol Dept Med Surg &

    Expt Sci Vle S Pietro 43-B I-07100 Sassari Italy;

    Univ Sassari Sect Pharmacol Dept Med Surg &

    Expt Sci Vle S Pietro 43-B I-07100 Sassari Italy;

    Univ Cagliari Dept Life &

    Environm Sci Univ Campus I-09042 Cagliari Italy;

    Univ Sassari Sect Pharmacol Dept Med Surg &

    Expt Sci Vle S Pietro 43-B I-07100 Sassari Italy;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 中毒及化学性损害;
  • 关键词

    Alcohol oxidase; Ethanol; Biosensor; Microvibration sensor; Voluntary ethanol intake; Sprague Dawley rats; Telemetry;

    机译：醇氧化酶;乙醇;生物传感器;微纤维传感器;自愿乙醇摄入;Sprague Dawley大鼠;遥测;

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