...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Allergy >Meat allergy associated with galactosyl‐α‐(1,3)‐galactose (α‐Gal)—Closing diagnostic gaps by anti‐α‐Gal IgE immune profiling
【24h】

Meat allergy associated with galactosyl‐α‐(1,3)‐galactose (α‐Gal)—Closing diagnostic gaps by anti‐α‐Gal IgE immune profiling

机译：肉体过敏与半乳糖基-α-（1,3） - 用抗α-GAL IGE免疫分析 - 杀菌（α-GAL） - 将诊断间隙染色

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract Background Glycoproteins and glycolipids of some mammalian species contain the disaccharide galactosyl‐α‐(1,3)‐galactose (α‐Gal). It is known that α‐Gal is immunogenic in humans and causes glycan‐specific IgG and also IgE responses with clinical relevance. α‐Gal is part of the IgE‐reactive monoclonal therapeutic antibody cetuximab (CTX) and is associated with delayed anaphylaxis to red meat. In this study, different α‐Gal‐containing analytes are examined in singleplex and multiplex assays to resolve individual sensitization patterns with IgE against α‐Gal. Methods Three serum groups, α‐Gal‐associated meat allergy ( MA ) patients, idiopathic anaphylaxis ( IA ) patients with suspected MA, and non‐meat‐allergic healthy control individuals ( HC ), were analyzed via singleplex allergy diagnostics and a newly established immunoblot diagnostic system. The new dot blot detection system resolved individual IgE sensitization profiles for α‐Gal‐containing analytes CTX, bovine thyroglobulin (Bos d TG), and human serum albumin ( HSA )‐conjugated α‐Gal. Results Singleplex allergy diagnostics using the α‐Gal analytes CTX and Bos d TG confirms the history of MA patients in 91% and 88% of the cases, respectively. A novel dot blot‐based assay system for the detection of IgE against α‐Gal reveals individual IgE sensitization profiles for α‐Gal‐containing analytes. An α‐Gal‐associated IgE cross‐reactivity profile (IgE against CTX, Bos d TG, and HSA ‐α‐Gal) was identified, which is associated with MA. Conclusions Detection of individual sensitization patterns with different α‐Gal‐containing analytes provides the basis for an individual allergy diagnosis for α‐Gal‐sensitized patients. Higher amounts of α‐Gal in pork and beef innards compared to muscle meat as indicated by a higher staining intensity are a plausible explanation for the difference in allergic symptom severity.
机译：摘要背景糖蛋白和一些哺乳动物物种的糖脂蛋白含有二糖半乳糖基-α-（1,3） - 糖（α-加仑）。众所周知，α-加仑在人体中是免疫原性，并导致甘草特异性的IgG以及临床相关性的IgE反应。 α-GAL是IgE反应性单克隆治疗抗体的一部分，其与红肉的延迟过敏和延迟的过敏症相关。在该研究中，在单行和多重测定中检查不同的含α-Gal的分析物，以解决与α-GAL的IgE的单独敏化图案。方法采用单侧过敏诊断分析三种血清群，α-丙关联肉体过敏（MA），特发性过敏性（IA）患者，具有非肉类过敏性健康对照个体（HC）的患者，并进行了新建立免疫抑制诊断系统。新的点印迹检测系统解决了含α-Gal的分析CTX，牛甲基蛋白（BOS D Tg）和人血清白蛋白（HSA） - 缀合的α-Gal的单独的IgE致敏曲线。结果使用α-gal分析型CTX和BOS D TG的单倒入过敏诊断分别在91％和88％的病例中确认了MA患者的历史。用于检测IgE对抗α-GAL的新型点印迹测定系统揭示了含α-加仑的分析物的个体IgE致敏曲线。鉴定了α-加仑相关的IgE交叉反应性曲线（IgE针对CTX，BOS D Tg和HSA-α-GAL），其与MA相关。结论含有不同α-Gal的分析物的单个敏化模式的检测为α-加仑致敏患者的个体过敏诊断提供了基础。与肌肉肉相比，猪肉和牛肉内的量较多的α-gal，如较高的染色强度所示是对过敏性症状严重程度的差异的合理解释。

著录项

  • 来源
    《Allergy》 |2018年第1期|共13页
  • 作者

    Jappe U.; Minge S.; Kreft B.; Ludwig A.; Przybilla B.; Walker A.; Varga R.; Seidel P.; Biedermann T.; Anemüller W.; Kromminga A.; Ru?ff F.; Merk H.; Wagner N.; Treudler R.; Worm M.; Waldmann I.; Saloga J.; Becker W. M.; Goldmann T.; Platts‐Mills T. A.; Homann A.;

  • 作者单位

    Division of Clinical and Molecular AllergologyMember of the German Center for Lung Research (DZL;

    Division of Clinical and Molecular AllergologyMember of the German Center for Lung Research (DZL;

    Department of Dermatology and VenereologyMartin‐Luther‐University Halle‐WittenbergHalle (Saale;

    Department of Dermatology and AllergologyKlinikum AugsburgAugsburg Germany;

    Department of DermatologyLudwig‐Maximilians UniversityMunich Germany;

    Department of DermatologyLudwig‐Maximilians UniversityMunich Germany;

    Department of DermatologyLudwig‐Maximilians UniversityMunich Germany;

    Department of DermatologyUniversity of TübingenTübingen Germany;

    Department of DermatologyUniversity of TübingenTübingen Germany;

    Department of DermatologyUniversity of LübeckLübeck Germany;

    IPM BiotechHamburg Germany;

    Department of DermatologyLudwig‐Maximilians UniversityMunich Germany;

    Department of DermatologyRWTH Aachen UniversityAachen Germany;

    Department of DermatologyKlinikumDarmstadt Germany;

    Department of Dermatology Venereology and AllergologyUniversity of LeipzigLeipzig Germany;

    Department of Dermatology and AllergyAllergy Center CharitéBerlin Germany;

    Dermatology &

    AllergologySt. Bernward KlinikHildesheim Germany;

    Department of DermatologyUniversity of MainzMainz Germany;

    Division of Clinical and Molecular AllergologyMember of the German Center for Lung Research (DZL;

    Pathology of the University Medical Center Schleswig‐Holstein (UKSH)Member of the German Center for;

    University of Virginia Asthma and Allergic Diseases CenterCharlottesville USA;

    Division of Clinical and Molecular AllergologyMember of the German Center for Lung Research (DZL;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医学免疫学;
  • 关键词

    alpha‐Gal; bovine thyroglobulin; cetuximab; delayed anaphylaxis; meat allergy;

    机译：alpha-gal;牛甲状腺球蛋白;西妥昔单抗;延迟过敏;肉体过敏;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号