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首页> 外文期刊>Andrologia >Cytosolic phospholipase A 2 2 and F 2 2 isoprostanes are involved in semen quality and human infertility—A study on leucocytospermia, varicocele and idiopathic infertility
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Cytosolic phospholipase A 2 2 and F 2 2 isoprostanes are involved in semen quality and human infertility—A study on leucocytospermia, varicocele and idiopathic infertility

机译：细胞溶质磷脂酶A 2和F 2 2异己醇参与精液质量和人类不孕症 - 对白细胞症，Varicocele和特发性不孕症的研究

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Abstract Phospholipase A 2 (PLA 2 ) is involved in eicosanoid release, and F 2 ‐isoprostanes (F 2 ‐IsoPs), as free radical‐generated eicosanoids released by PLA 2 , are indicators of oxidative stress in different human conditions. This study investigated the interplay between cytosolic PLA 2 (cPLA 2 ), F 2 ‐IsoPs and sperm features in male infertility, when the involvement of oxidative stress has been reported. Semen evaluation was performed following WHO guidelines, sperm ultrastructure was detected by transmission electron microscopy indicating a fertility index, and the percentages of sperm immaturity, apoptosis and necrosis. In sperm cells and seminal plasma, cPLA 2 levels were determined by immunological method, whereas F 2 ‐IsoPs by mass spectrometry. Sperm concentration, morphology, vitality and fertility index values were significantly lower in infertile groups compared with fertile men. An increase in sperm apoptosis and necrosis ( p ??.01), apoptosis ( p ??.01) and immaturity ( p ??.001) was detected in leucocytospermia, idiopathic infertility and varicocele, respectively. Seminal cPLA 2 showed the highest value in varicocele group ( p ??.05), whereas seminal F 2 ‐IsoPs increased in varicocele ( p ??.001) and leucocytospermia ( p ??.05) groups. In the whole population, F 2 ‐IsoP and cPLA 2 levels were positively correlated ( p ??.05). On the contrary, F 2 ‐IsoPs and cPLA 2 were not significantly different when investigated in sperm cells. Our data indicate that fatty acid oxidation/metabolism plays a role in different male reproductive pathological conditions.
机译：摘要磷脂酶A 2（PLA 2）涉及逐渐释放，并且F 2 - 己烷（F 2-USOPS）作为PLA 2释放的自由基产生的大藻醇，是氧化应激在不同人体条件下的指示剂。该研究在报道氧化应激的累积时，研究了胞岩PLA 2（CPLA 2），F 2-USOPS和精子特征之间的相互作用。通过透射电子显微镜检测到谁的指导原则进行精液评估，表明生育指数，精子不成熟，细胞凋亡和坏死的百分比检测到精子超微结构。在精子细胞和精体等离子体中，通过免疫学法测定CPLA 2水平，而F 2 - 通过质谱法测定。与肥沃的男性相比，不孕群体的精子浓度，形态，活力和生育率指数值显着降低。精子细胞凋亡和坏死（p≤01）的增加，分别在白细胞症，特发性不孕症和Varicocele中检测到细胞凋亡（p≤01），细胞凋亡（p≤01）和不饱和度（p？＆ 001）。精髓果皮2显示出Varicocele组的最高值（p？＆Δ05），而精液F 2 - 在Varicocele（p≤001）和白细胞肌瘤（p≤05）中增加（p？＆ 05）组。在整个人口中，F 2 -ISOP和CPLA 2水平呈正相关（P？＆ 05）。相反，在精子细胞中研究时，F 2-USOP和CPLA 2没有显着差异。我们的数据表明脂肪酸氧化/代谢在不同的男性生殖病理条件下起作用。

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