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首页> 外文期刊>Acta Physiologiae Plantarum >Somatic embryogenesis-mediated plant regeneration of Eclipta alba (L.) Hassk. and its conservation through synthetic seed technology
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Somatic embryogenesis-mediated plant regeneration of Eclipta alba (L.) Hassk. and its conservation through synthetic seed technology

机译：体细胞胚胎发生介导的Eclipta Alba（L.）Hassk的植物再生。 通过合成种子技术保护

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摘要

Plant regeneration by means of somatic embryogenesis has been standardized for the first time in Eclipta alba (L.) Hassk. Explants like nodal segment, shoot tip, and leaf were tested on Murashige and Skoog medium supplemented with picloram, 2,4-dichlorophenoxyacetic acid, or -naphthalene acetic acid for callus induction. Nodal segment exhibited the maximum response (92.80%) to callus induction on culture medium-containing 2.0 mgl(-1) picloram. Furthermore, highest rate (88.40%) and number (34.83) of somatic embryo induction were found on medium fortified with 2.0 mgl(-1) picloram, 0.5 mgl(-1) thidiazuron, and 0.25 mgl(-1) abscisic acid. Influence of abscisic acid on the somatic embryo regeneration was also tested with the highest frequency (95.50%) attained on medium-containing 0.75 mgl(-1) N-6-benzyladenine and 0.5 mgl(-1) abscisic acid. Somatic embryo of torpedo stage was efficiently encapsulated with 2.5% sodium alginate and 75mM calcium chloride, and exhibited 93.33% germination rate. The study revealed that storage at low temperature (8 degrees C) gave superior results where 86.67% of the synthetic seeds sprouted even after 60days with a decline to 46.67% in 90days post-storage. The rate of germination at 25 degrees C had been constantly low and failed to sprout after 75days. The acclimatization of the regenerated plantlets was successfully taken place in garden soil, sand, and vermicompost in the ratio of 1:1:1 with 95% survival rate.
机译：植物再生通过体细胞胚胎发生在Eclipta Alba（L.）Hassk中首次标准化。萼片等植物如节点，射尖和叶子在补充有米洛仑，2,4-二氯苯乙烯酸乙酸或萘乙酸的Murashige和Skoog培养基上进行测试，用于愈伤组织诱导。节点段显示出含培养基含有2.0mg1（-1）氨基氯蛋白的愈伤组织诱导的最大反应（92.80％）。此外，在强化2.0mgL（-1）氨基氯仑，0.5mg（-1）吡吡氏菌和0.25mg（-1）脱落酸的培养基中，发现了体细胞胚诱导的最高速率（88.40％）和数量（34.83）。脱落酸对体细胞胚再生的影响也用最高频率（95.50％）进行，含培养基0.75mg（-1）N-6-苄基腺苷和0.5mg（-1）脱落酸。鱼雷阶段的体细胞胚状物用2.5％海藻酸钠和75mM氯化钙有效地包封，并发芽萌发率为93.33％。该研究表明，低温（8摄氏度）的储存使得86.67％的合成种子甚至在60天后发芽，在储存后90天内下降至46.67％。 25摄氏度的发芽率经常低，75天后未能发芽。在园林土壤，沙子和蛭石中成功地将再生植株的适应性成功地在1：1：1的比例中成功地进行了95％的存活率。

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