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Seroprevalence of Antibodies against Porcine Teschovirus 1 in Lithuania

机译:在立陶宛针对猪细小病毒1抗体的血清阳性。

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The objective of the present work was to study the serological status and epidemiologicalsituation of porcine teschovirus 1 (PTV-1) on swine farms in different Lithuanian regions by usingvirus neutralization test and virus isolation. Clinical, epidemiological, serological and virological examinations were performed for the diagnosis of Teschen disease (porcine enterovirus encephalomyelitis, TE). Swine blood serum samples (n = 1680) werecollected on 28 farms (60 samples per farm) of 8 regions in 2003. For virus isolation 29 samples of pathological material were taken from the brains of pigs (2 - 4 months of age) that died suddenly on seven swine farms in five regions. Epidemiological and clinical examination revealed no signs of porcine enterovirusencephalomyelitis on 32 Lithuanian swine farms. PTV-1 was not isolated and identified in thePK-15 cell culture. Antibodies against PTV-1, detected by using VNT, were found on allinvestigated farms and in all age groups. Negative serum (2.3%, 39 from 1680) samples were foundin 15.5% (16 from 103) of 2-4-month-old pigs and in 4.7% (23 from 494) of 4-6-month-old ones.The positive serological and negative epidemiological, clinical and virological results suggest thatless virulent or avirulent PTV-1 strains were spread on Lithuanian swine farms.
机译:本研究的目的是通过病毒中和试验和病毒分离来研究立陶宛地区不同猪场的猪破伤风病毒1(PTV-1)的血清学状况和流行病学情况。进行了临床,流行病学,血清学和病毒学检查,以诊断Teschen病(猪肠病毒脑脊髓炎,TE)。 2003年在8个地区的28个农场(每个农场60个样本)中采集了猪血清样本(n = 1680)。为进行病毒分离,从死去的猪(2-4月龄)的大脑中提取了29个病理物质样本突然出现在五个地区的七个养猪场。流行病学和临床检查显示,立陶宛32个养猪场没有猪肠病毒性脑脊髓炎的迹象。在PK-15细胞培养物中未分离和鉴定出PTV-1。通过VNT检测到的针对PTV-1的抗体已在所有调查农场和所有年龄组中发现。在2-4个月大的猪中,有15.5%的血清(2.3%,来自1680中的39个)被检出,而4-6个月大的猪中则有4.7%(494中的23个)的血清中被检出。血清学和阴性流行病学,临床和病毒学结果表明,立陶宛养猪场没有传播任何毒性强或无毒的PTV-1菌株。

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